Antibody production in Japanese eels, Anguilla japonica Temminck & Schlegel

Adult Japanese eels, Anguilla japonica Temminck & Schlegel, (200–250 g, 45–55 cm) were immunized by intramuscular injection with goat IgG. After 5 weeks, eel immunoglobulin (Ig) was purified using affinity chromatography. The purified eel Ig was used to immunize rabbits to produce anti‐eel Ig an...

Full description

Saved in:
Bibliographic Details
Published inJournal of fish diseases Vol. 20; no. 3; pp. 195 - 200
Main Authors Hung, H-W, Lo, C-F, Tseng, C-C, Kou, G-H
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Science Ltd 01.05.1997
Subjects
Anguilla japonica
Marine
Online AccessGet full text

Cover

More Information
Summary:Adult Japanese eels, Anguilla japonica Temminck & Schlegel, (200–250 g, 45–55 cm) were immunized by intramuscular injection with goat IgG. After 5 weeks, eel immunoglobulin (Ig) was purified using affinity chromatography. The purified eel Ig was used to immunize rabbits to produce anti‐eel Ig antibody. The highest antibody ELISA value in eels was reached 3 weeks after initial immunization with goat IgG, and then gradually decreased. The antibody could still be detected at 140 days post‐immunization. The optimal temperature for antibody production was 30°C. Freund’s complete adjuvant and secondary immunization both increased antibody production in eels.
Bibliography:istex:F5EFADD9E54C1B1FE309C62FF6E9FFA4FB2BA1FA
ark:/67375/WNG-D223KS4W-P
ArticleID:JFD290
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:0140-7775
1365-2761
DOI:10.1046/j.1365-2761.1997.00290.x