Bacterial extracellular vesicles in the microbiome of first-pass meconium in newborn infants

• Published in: Pediatric research Vol. 93; no. 4; pp. 887 - 896
• Main Authors: Turunen, Jenni, Tejesvi, Mysore V., Suokas, Marko, Virtanen, Nikke, Paalanne, Niko, Kaisanlahti, Anna, Reunanen, Justus, Tapiainen, Terhi
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.03.2023; Nature Publishing Group
• Subjects: Anti-Bacterial Agents; Antibiotics; Bacteria - genetics; Basic Science; Basic Science Article; Childbirth & labor; Extracellular vesicles; Feces; Female; Humans; Infant; Infant, Newborn; Meconium - microbiology; Medicine; Medicine & Public Health; Microbiota; Newborn babies; Pediatric Surgery; Pediatrics; Pregnancy; RNA, Ribosomal, 16S - genetics
• ISSN: 0031-3998; 1530-0447
• DOI: 10.1038/s41390-022-02242-1

Background Bacterial extracellular vesicles (EVs) are more likely to cross biological barriers than whole-cell bacteria. We previously observed EV-sized particles by electron microscopy in the first-pass meconium of newborn infants. We hypothesized that EVs may be of bacterial origin and represent a novel entity in the human microbiome during fetal and perinatal periods. Methods We extracted EVs from first-pass meconium samples of 17 newborn infants and performed bacterial 16S rRNA gene sequencing of the vesicles. We compared the EV content from the meconium samples of infants based on the delivery mode, and in vaginal delivery samples, based on the usage of intrapartum antibiotics. Results We found bacterial EVs in all first-pass meconium samples. All EV samples had bacterial RNA. Most of the phyla present in the samples were Firmicutes (62%), Actinobacteriota (18%), Proteobacteria (10%), and Bacteroidota (7.3%). The most abundant genera were Streptococcus (21%) and Staphylococcus (17%). The differences between the delivery mode and exposure to antibiotics were not statistically significant. Conclusions Bacterial EVs were present in the first-pass meconium of newborn infants. Bacterial EVs may represent an important novel feature of the gut microbiome during fetal and perinatal periods. Impact We show that bacterial extracellular vesicles are present in the microbiome of first-pass meconium in newborn infants. This is a novel finding. To our knowledge, this is the first study to report the presence of bacterial extracellular vesicles in the gut microbiome during fetal and perinatal periods. This finding is important because bacterial extracellular vesicles are more likely to cross biological barriers than whole-cell bacteria. Thus, the early gut microbiome may potentially interact with the host through bacterial EVs.