Gold nanoparticles covalently assembled onto vesicle structures as possible biosensing platform

• Published in: Beilstein journal of nanotechnology Vol. 7; no. 1; pp. 655 - 663
• Main Authors: Barroso, M Fátima, Luna, M Alejandra, Tabares, Juan S Flores, Delerue-Matos, Cristina, Correa, N Mariano, Moyano, Fernando, Molina, Patricia G
• Format: Journal Article
• Language: English
• Published: Germany Beilstein-Institut zur Föerderung der Chemischen Wissenschaften 2016; Beilstein-Institut
• Subjects: Antigens; Bilayers; Biosensors; Covalence; covalently decorated vesicles; Full Research Paper; Glycerol; Gold; gold nanoparticles; Immunosensors; immunosensors design; Morphology; Nanomaterials; Nanoparticles; Nanoscience; Nanotechnology; Self-assembly; Spectrum analysis; synthesis; Vesicles; Voltammetry; synthesis; immunosensors design; covalently decorated vesicles; gold nanoparticles
• ISSN: 2190-4286; 2190-4286
• DOI: 10.3762/bjnano.7.58

In this contribution a strategy is shown to covalently immobilize gold nanoparticles (AuNPs) onto vesicle bilayers with the aim of using this nanomaterial as platform for the future design of immunosensors. A novel methodology for the self-assembly of AuNPs onto large unilamellar vesicle structures is described. The vesicles were formed with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1-undecanethiol (SH). After, the AuNPs photochemically synthesized in pure glycerol were mixed and anchored onto SH-DOPC vesicles. The data provided by voltammetry, spectrometry and microscopy techniques indicated that the AuNPs were successfully covalently anchored onto the vesicle bilayer and decorated vesicles exhibit a spherical shape with a size of 190 ± 10 nm. The developed procedure is easy, rapid and reproducible to start designing a possible immunosensor by using environmentally friendly procedures.