Plateletworks ® as a Point-of-Care Test for ASA Non-Sensitivity

• Published in: Journal of personalized medicine Vol. 11; no. 8; p. 813
• Main Authors: Khan, Hamzah, Jain, Shubha, Gallant, Reid C, Syed, Muzammil H, Zamzam, Abdelrahman, Al-Omran, Mohammed, Rand, Margaret L, Ni, Heyu, Abdin, Rawand, Qadura, Mohammad
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20.08.2021; MDPI
• Subjects: Anemia; antiplatelet; Aspirin; atherosclerosis; Blood platelets; Cardiovascular disease; Collagen; Coronary vessels; Diabetes; Hematology; Hypertension; Hypothesis testing; Kidney diseases; Leukopenia; light transmission aggregometry; non-sensitivity; Patients; Platelet aggregation; Precision medicine; resistance; Sensitivity analysis; Standard deviation; Statistical analysis; Thrombocytopenia; United States > US; China; atherosclerosis; non-sensitivity; light transmission aggregometry; Plateletworks; aspirin; antiplatelet; resistance
• ISSN: 2075-4426; 2075-4426
• DOI: 10.3390/jpm11080813

Aspirin (ASA) therapy is proven to be effective in preventing adverse cardiovascular events; however, up to 30% of patients are non-sensitive to their prescribed ASA dosage. In this pilot study, we demonstrated, for the first time, how ASA non-sensitivity can be diagnosed using Plateletworks , a point-of-care platelet function test. Patients prescribed 81 mg of ASA were recruited in a series of two successive phases-a discovery phase and a validation phase. In the discovery phase, a total of 60 patients were recruited to establish a cut-off point (COP) for ASA non-sensitivity using Plateletworks . Each sample was simultaneously cross-referenced with a light transmission aggregometer (LTA). Our findings demonstrated that >52% maximal platelet aggregation using Plateletworks had a sensitivity, specificity, and likelihood ratio of 80%, 70%, and 2.67, respectively, in predicting ASA non-sensitivity. This COP was validated in a secondary cohort of 40 patients prescribed 81 mg of ASA using Plateletworks and LTA. Our data demonstrated that our established COP had a 91% sensitivity and 69% specificity in identifying ASA non-sensitivity using Plateletworks . In summary, Plateletworks is a point-of-care platelet function test that can appropriately diagnose ASA non-sensitive patients with a sensitivity exceeding 80%.