Comparison between Roche Integra 400 plus and Abbott Architect ci8200 in Alanine aminotransferase assay

• Published in: Practical laboratory medicine Vol. 15; p. e00121
• Main Authors: Lamrabat, Somiya, Lyagoubi, Amina, Bouayadi, Ouardia, Elmalki, Jalila, Elkouche, Khadija, Bensalah, Mohammed, Choukri, Mohammed
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.2019; Elsevier
• Subjects: Abbott diagnostic; Alanine aminotransferase; Comparison; Concordance; Roche diagnostic; Roche diagnostic; Alanine aminotransferase; Abbott diagnostic; Comparison; Concordance
• ISSN: 2352-5517; 2352-5517
• DOI: 10.1016/j.plabm.2019.e00121

The aim of this work is to present the results of a comparative study between the ALT assay on Integra 400 plus Roche Diagnostic versus Architect ci8200 of Abbott Diagnostic. A total of 200 patients hospitalized in the various departments of the university hospital Mohammed VI of Oujda were prospectively tested on two systems: Abbott Architect ci8200 and Roche Integra 400 plus. Both analyzers use the spectrophotometric technique by coupling the transamination reaction to the oxidation-reduction reaction at NAD. The agreement of the results between the different techniques was evaluated using the Bland-Altman difference diagram and the Passing-Bablok and Deming regression line. There was a high concordance between the two assays: the equation of the Passing-Bablok line is YArchitect ​= ​−0,5625 ​+ ​0,9917 XIntegra with a correlation coefficient r2 = 0.999. The Bland-Altman diagram shows that the mean bias between the two methods is of the order of 1.1 IU/L and the difference between the ALT measurements by Architect and Integra is in the range of −1.4 to 3.6. Our study shows a high correlation of the ALT assay results between the architect ci8200 and Integra 400 plus.