Magnetic Cationic Amylose Nanoparticles Used to Deliver Survivin-Small Interfering RNA for Gene Therapy of Hepatocellular Carcinoma In Vitro

Amylose is a promising nanocarrier for gene delivery in terms of its good biocompatibility and high transfection efficiency. Small interfering RNA against survivin (survivin-siRNA) can cause tumor apoptosis by silencing a hepatocellular carcinoma (HCC)-specific gene at the messenger RNA level. In th...

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Published inNanomaterials (Basel, Switzerland) Vol. 7; no. 5; p. 110
Main Authors Wu, Zhuo, Xu, Xiao-Lin, Zhang, Jun-Zhao, Mao, Xu-Hong, Xie, Ming-Wei, Cheng, Zi-Liang, Lu, Lie-Jing, Duan, Xiao-Hui, Zhang, Li-Ming, Shen, Jun
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 11.05.2017
MDPI
Subjects
Amylose
Apoptosis
Biocompatibility
Cations
Cytotoxicity
Folic acid
Gene therapy
Gene transfer
Hepatocellular carcinoma
In vitro methods and tests
Iron
Iron oxides
Liver cancer
magnetic resonance imaging
mRNA
Nanoparticles
Ribonucleic acid
RNA
siRNA
small interfering RNA
superparamagnetic iron oxide nanoparticles
Survivin
Toxicity
Transfection
magnetic resonance imaging
amylose
small interfering RNA
superparamagnetic iron oxide nanoparticles
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Summary:Amylose is a promising nanocarrier for gene delivery in terms of its good biocompatibility and high transfection efficiency. Small interfering RNA against survivin (survivin-siRNA) can cause tumor apoptosis by silencing a hepatocellular carcinoma (HCC)-specific gene at the messenger RNA level. In this study, we developed a new class of folate-functionalized, superparamagnetic iron oxide (SPIO)-loaded cationic amylose nanoparticles to deliver survivin-siRNA to HCC cells. The cellular uptake of nanocomplexes, cytotoxicity, cell apoptosis, and gene suppression mediated by siRNA-complexed nanoparticles were tested. The results demonstrated that folate-functionalized, SPIO-loaded cationic amylose nanoparticles can mediate a specific and safe cellular uptake of survivin-siRNA with high transfection efficiency, resulting in a robust survivin gene downregulation in HCC cells. The biocompatible complex of cationic amylose could be used as an efficient, rapid, and safe gene delivery vector. Upon SPIO loading, it holds a great promise as a theranostic carrier for gene therapy of HCC.
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These authors contribute equally to this work.
ISSN:2079-4991
2079-4991
DOI:10.3390/nano7050110