Adult plant stem rust resistance in durum wheat Glossy Huguenot: mapping, marker development and validation

• Published in: Theoretical and applied genetics Vol. 135; no. 5; pp. 1541 - 1550
• Main Authors: Mago, Rohit, Chen, Chunhong, Xia, Xiaodi, Whan, Alex, Forrest, Kerrie, Basnet, Bhoja R., Perera, Geetha, Chandramohan, Sutha, Randhawa, Mandeep, Hayden, Matthew, Bansal, Urmil, Huerta-Espino, Julio, Singh, Ravi P., Bariana, Harbans, Lagudah, Evans
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.05.2022; Springer; Springer Nature B.V
• Subjects: Agricultural research; Agriculture; Biochemistry; Biomedical and Life Sciences; Biotechnology; Chromosome mapping; Chromosomes; Control; Cultivars; Disease resistance; Diseases and pests; Durum wheat; Gene mapping; Genes; Genetic aspects; genomics; Genotyping; inbred lines; Inbreeding; Life Sciences; loci; mature plants; Methods; Original Article; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant immunology; Polymorphism; Population; Quantitative trait loci; resistance genes; Rust diseases; Single-nucleotide polymorphism; Stem rust; Triticum durum; Virulence; Australia
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-022-04052-9

Key message Adult plant stem rust resistance locus, QSrGH.cs-2AL , was identified in durum wheat Glossy Huguenot and mendelised as Sr63 . Markers closely linked with Sr63 were developed. An F 3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F 6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F 6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F 6 recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F 6:7 population was developed through single seed descent method. F 7 and F 8 RILs were tested along with the parents at different locations. The F 6 individual plants and both parents were genotyped using the 90 K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B ( QSrGH.cs-1BL ) and 2A ( QSrGH.cs-2AL ) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL . Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63 . SNP markers associated with Sr63 were converted to kompetetive allele-specific PCR (KASP) assays and were validated on a set of durum cultivars.