Stable DNA Aptamer-Metal-Organic Framework as Horseradish Peroxidase Mimic for Ultra-Sensitive Detection of Carcinoembryonic Antigen in Serum

Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. For CEA detection, a novel type of metal-organic framework (MOF) was prepared by grafting CEA aptamer-incorporated DNA tetrahedral (TDN) nanostructures into PCN-222 (Fe)-based MOF (referred as CEA -TDN-MOF colloid nanorods)....

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Bibliographic Details
Published inGels Vol. 7; no. 4; p. 181
Main Authors Sha, Lingjun, Zhu, Mingcong, Lin, Fuqing, Yu, Xiaomeng, Dong, Langjian, Wu, Licheng, Ding, Rong, Wu, Shuai, Xu, Jingjing
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 25.10.2021
MDPI
Subjects
Antibodies
Antigens
Biocompatibility
Biosensors
carcinoembryonic antigen
Catalytic oxidation
colloid nanorods
Colon
colorimetric detection
Colorimetry
DNA tetrahedron nanostructure
Iron
Ligands
Metal-organic frameworks
Nanoparticles
Nanorods
Peptides
Peroxidase
Phosphorylation
Porphyrins
Target detection
Tumors
Zirconium
colorimetric detection
colloid nanorods
DNA tetrahedron nanostructure
carcinoembryonic antigen
catalytic oxidation
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Summary:Carcinoembryonic antigen (CEA) is an important broad-spectrum tumor marker. For CEA detection, a novel type of metal-organic framework (MOF) was prepared by grafting CEA aptamer-incorporated DNA tetrahedral (TDN) nanostructures into PCN-222 (Fe)-based MOF (referred as CEA -TDN-MOF colloid nanorods). The synthesized CEA -TDN-MOF is a very stable detection system due to the vertex phosphorylated TDN structure at the interface, possessing a one-year shelf-life. Moreover, it exhibits a significant horseradish peroxidase mimicking activity due to the iron porphyrin ring, which leads to a colorimetric reaction upon binding toward antibody-captured CEA. Using this method, we successfully achieved the highly specific and ultra-sensitive detection of CEA with a limit of detection as low as 3.3 pg/mL. In addition, this method can detect and analyze the target proteins in clinical serum samples, effectively identify the difference between normal individuals and patients with colon cancer, and provide a new method for the clinical diagnosis of tumors, demonstrating a great application potential.
Bibliography:These authors contributed equally to this work.
ISSN:2310-2861
2310-2861
DOI:10.3390/gels7040181