Risk association, linkage disequilibrium and haplotype analyses of FASN rs4246445, rs2229425, rs2228305 and rs2229422 polymorphisms in overweight and obesity

• Published in: BioImpacts : BI Vol. 8; no. 3; pp. 159 - 165
• Main Authors: Chong, Eric Tzyy Jiann, Kuok, Shawn Shi Erh, Lee, Ping-Chin
• Format: Journal Article
• Language: English
• Published: Iran Tabriz University of Medical Sciences 01.01.2018
• Subjects: Adults; Age; Alcohol; Association analysis; Biochemistry; Blood; Body fat; Body mass index; Body weight; Deoxyribonucleic acid; Diabetes; DNA; DNA probes; Enzymes; Epidemiology; Equilibrium; Fatty acids; Fatty-acid synthase; Food; Gender; Gene expression; Genotyping; Haplotypes; Hemoglobin; Inflammation; Linkage analysis; Linkage disequilibrium; Lipids; Obesity; Original Research; Overweight; Population; single nucleotide polymorphisms; Single-nucleotide polymorphism; United States > US; Malaysia; Obesity; Fatty acid synthase; Single nucleotide polymorphisms; Overweight
• ISSN: 2228-5660; 2228-5652; 2228-5660
• DOI: 10.15171/bi.2018.18

Obesity is commonly linked up with several life-threatening diseases. This study aims to investigate the association of fatty acid synthase ( ) rs4246445, rs2229425, rs2228305, and rs2229422 single nucleotide polymorphisms (SNPs) with the risk of overweight and obesity in the Malaysian population. Blood samples were collected from 1030 individuals who were grouped into normal, overweight, and obese categories. Blood biochemistry test and lipid profiling were performed and genomic DNA was extracted. Genotyping was performed using hydrolysis probes and odd ratio with 95% CI was calculated for risk association analysis. Linkage disequilibrium and haplotypes analyses were carried out using SHEsis software. We found that the hemoglobin and white blood cell counts were significantly high in the obese subjects. There is a lack of evidence to link the SNPs with the risk of overweight and obesity in the population. All 4 SNPs were seemed to be in linkage equilibrium. Five common haplotypes were identified in this study but none of them was significantly associated with overweight and obesity in the population. Our findings suggest a lack of evidence to associate the rs4246445, rs2229425, rs2228305, and rs2229422 SNPs with the risk of overweight and obesity in the Malaysian population. All 4 SNPs were independent of each other and not all identified haplotypes were significantly associated with overweight and obesity in this study.