The length of lipoteichoic acid polymers controls Staphylococcus aureus cell size and envelope integrity

• Published in: Journal of bacteriology Vol. 202; no. 16
• Main Authors: Hesser, Anthony R, Matano, Leigh M, Vickery, Christopher R, Wood, B McKay, Santiago, Ace George, Morris, Heidi G, Do, Truc, Losick, Richard, Walker, Suzanne
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.08.2020
• Subjects: Abnormalities; Amides; Antibiotics; Bacteriology; Cell division; Cell growth; Cell membranes; Cell size; Cell walls; Defects; Deletion mutant; Lipoteichoic acid; Mutants; Mutation; Opportunist infection; Penicillin; Peptidoglycans; Phenotypes; Polymers; Spotlight; Staphylococcus aureus; β-Lactam antibiotics
• ISSN: 0021-9193; 1098-5530
• DOI: 10.1128/jb.00149-20

The opportunistic pathogen is protected by a cell envelope that is crucial for viability. In addition to peptidoglycan, lipoteichoic acid (LTA) is an especially important component of the cell envelope. LTA is an anionic polymer anchored to a glycolipid in the outer leaflet of the cell membrane. It was known that deleting the gene for UgtP, the enzyme that makes this glycolipid anchor, causes cell growth and division defects. In , growth abnormalities from the loss of have been attributed to both the absence of the encoded protein and to the loss of its products. Here, we show that growth defects in deletion mutants are due to the long, abnormal LTA polymer that is produced when the glycolipid anchor is missing from the outer leaflet of the membrane. Dysregulated cell growth leads to defective cell division, and these phenotypes are corrected by mutations in the LTA polymerase, , that reduce polymer length. We also show that mutants with long LTA are sensitized to cell wall hydrolases, beta-lactam antibiotics, and compounds that target other cell envelope pathways. We conclude that control of LTA polymer length is important for physiology and promotes survival under stressful conditions, including antibiotic stress. Methicillin-resistant (MRSA) is a common cause of community- and hospital-acquired infections and is responsible for a large fraction of deaths caused by antibiotic-resistant bacteria. is surrounded by a complex cell envelope that protects it from antimicrobial compounds and other stresses. Here we show that controlling the length of an essential cell envelope polymer, lipoteichoic acid, is critical for controlling cell size and cell envelope integrity. We also show that genes involved in LTA length regulation are required for resistance to beta-lactam antibiotics in MRSA. The proteins encoded by these genes may be targets for combination therapy with an appropriate beta-lactam.