A vertebral skeletal stem cell lineage driving metastasis

• Published in: Nature (London) Vol. 621; no. 7979; pp. 602 - 609
• Main Authors: Sun, Jun, Hu, Lingling, Bok, Seoyeon, Yallowitz, Alisha R., Cung, Michelle, McCormick, Jason, Zheng, Ling J., Debnath, Shawon, Niu, Yuzhe, Tan, Adrian Y., Lalani, Sarfaraz, Morse, Kyle W., Shinn, Daniel, Pajak, Anthony, Hammad, Mohammed, Suhardi, Vincentius Jeremy, Li, Zan, Li, Na, Wang, Lijun, Zou, Weiguo, Mittal, Vivek, Bostrom, Mathias P. G., Xu, Ren, Iyer, Sravisht, Greenblatt, Matthew B.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.09.2023; Nature Publishing Group
• Subjects: 13/100; 14/1; 631/532; 631/67/322/803; Adipocytes; Biomarkers; Bone growth; Bones; Breast cancer; Breast Neoplasms - pathology; Cartilage; Cell Differentiation; Cell Lineage; Cell Self Renewal; Cell surface; Differentiation; Humanities and Social Sciences; Humans; Labeling; Long bone; Metastases; Metastasis; multidisciplinary; Neoplasm Metastasis - pathology; Osteoblasts - cytology; Osteoblasts - pathology; Osteogenesis; Physiology; Science; Science (multidisciplinary); Solid tumors; Spine - cytology; Spine - pathology; Stem cells; Stem Cells - cytology; Stem Cells - metabolism; Stem Cells - pathology; Surface markers; Transcription factors; Tropism; Vertebrae
• ISSN: 0028-0836; 1476-4687; 1476-4687
• DOI: 10.1038/s41586-023-06519-1

Vertebral bone is subject to a distinct set of disease processes from long bones, including a much higher rate of solid tumour metastases 1 – 4 . The basis for this distinct biology of vertebral bone has so far remained unknown. Here we identify a vertebral skeletal stem cell (vSSC) that co-expresses ZIC1 and PAX1 together with additional cell surface markers. vSSCs display formal evidence of stemness, including self-renewal, label retention and sitting at the apex of their differentiation hierarchy. vSSCs are physiologic mediators of vertebral bone formation, as genetic blockade of the ability of vSSCs to generate osteoblasts results in defects in the vertebral neural arch and body. Human counterparts of vSSCs can be identified in vertebral endplate specimens and display a conserved differentiation hierarchy and stemness features. Multiple lines of evidence indicate that vSSCs contribute to the high rates of vertebral metastatic tropism observed in breast cancer, owing in part to increased secretion of the novel metastatic trophic factor MFGE8. Together, our results indicate that vSSCs are distinct from other skeletal stem cells and mediate the unique physiology and pathology of vertebrae, including contributing to the high rate of vertebral metastasis. Vertebral osteoblasts in mouse and human are formed from a precursor skeletal stem cell population that is distinct from long bone skeletal stem cells in function, location and transcriptional programme.