Dongia mobilis gen. nov., sp. nov., a new member of the family Rhodospirillaceae isolated from a sequencing batch reactor for treatment of malachite green effluent

• Published in: International journal of systematic and evolutionary microbiology Vol. 60; no. Pt 12; pp. 2780 - 2785
• Main Authors: YING LIU, JIN, Jing-Hua, LIU, Ying-Hao, ZHOU, Yu-Guang, LIU, Zhi-Pei
• Format: Journal Article
• Language: English
• Published: Reading Society for General Microbiology 01.12.2010
• Subjects: Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; DNA, Bacterial - genetics; Fatty Acids - chemistry; Fundamental and applied biological sciences. Psychology; Microbiology; Molecular Sequence Data; Phylogeny; Rhodospirillaceae - classification; Rhodospirillaceae - genetics; Rhodospirillaceae - isolation & purification; RNA, Ribosomal, 16S - genetics; Rosaniline Dyes; Sequence Analysis, DNA; Sewage - microbiology; Systematics; Ubiquinone - chemistry; Rhodospirillales; New genus; Treatment; Sequencing batch reactor; Taxonomy; Bacteria; New species; Rhodospirillaceae; Rhodospirillineae
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.020347-0

A Gram-negative, strictly aerobic and heterotrophic, non-spore-forming bacterial strain, designated LM22(T), was isolated from activated sludge of a sequencing batch reactor for the treatment of malachite green effluent. Cells of strain LM22(T) were slightly curved to straight rods (0.3-0.5×0.6-1.0μm) and motile by a single polar flagellum. Strain LM22(T) was negative for oxidase and catalase activities and phototrophic growth. An internal membrane system and bacteriochlorophyll a were absent. Growth occurred at 20-40°C (optimum 30-35°C) and pH6.0-10.0 (optimum pH7.0-7.5). Strain LM22(T) did not require NaCl for growth and tolerated up to 2.0 % NaCl (optimum 0.5 %). The major ubiquinone was Q-10. The major fatty acids (>10 % of the total) were C(18 : 1)ω7c (32.9 %), C(19 : 0) cyclo ω8c (18.7 %), C(16 : 0) (12.1 %) and C(16 : 0) 2-OH (10.5 %). Phylogenetic analysis of 16S rRNA gene sequences showed that Inquilinus limosus AU0476(T) was the closest relative (90.4 % 16S rRNA gene sequence similarity). The DNA G+C content was 65.6 mol%. On basis of phenotypic, chemotaxonomic and phylogenetic data, strain LM22(T) was considered to represent a novel genus and species of the family Rhodospirillaceae, for which the name Dongia mobilis gen. nov., sp. nov. is proposed. The type strain of Dongia mobilis is LM22(T) (=CGMCC 1.7660(T) =JCM 15798(T)).