DNA damage-inducible gene, UNC5A, functions as a tumor-suppressor in bladder cancer

UNC5 receptors are putative tumor suppressors whose expressions are lost in some cancers, but the role of UNC5A during DNA damage in bladder cancer remains undefined. To investigate into the potential function of UNC5A in bladder cancer, we examined UNC5A expression with real-time RT-PCR and Western...

Full description

Saved in:
Bibliographic Details
Published inTumor biology Vol. 35; no. 7; pp. 6887 - 6891
Main Authors Zhu, Yuyan, Yu, Meng, Chen, Yifu, Wang, Yixia, Wang, Junyong, Yang, Chunming, Bi, Jianbin
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Netherlands 01.07.2014
Springer Nature B.V
Subjects
Apoptosis
Apoptosis - drug effects
Biomedical and Life Sciences
Biomedicine
Bladder cancer
Cancer Research
Cell Line, Tumor
Cisplatin - administration & dosage
DNA damage
DNA Damage - drug effects
Gene expression
Genes, Tumor Suppressor
Humans
Receptors, Cell Surface - genetics
Receptors, Cell Surface - metabolism
Research Article
Tumor Suppressor Protein p53 - genetics
Urinary Bladder Neoplasms - drug therapy
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - pathology
UNC5A
Tumor suppressor
Bladder cancer
DNA damage
Online AccessGet full text

Cover

More Information
Summary:UNC5 receptors are putative tumor suppressors whose expressions are lost in some cancers, but the role of UNC5A during DNA damage in bladder cancer remains undefined. To investigate into the potential function of UNC5A in bladder cancer, we examined UNC5A expression with real-time RT-PCR and Western blotting in bladder cancer specimens and analyzed the effects of chemotherapeutic drug on the expression level of UNC5A and knocking down of UNC5A on chemotherapeutic drug-mediated cell death. In this current study, we found low expression of UNC5A in bladder cancer, an effective induction of UNC5A by cisplatin in bladder cancer cell lines with wt p53, and a significant reduction of cisplatin-mediated cell death following silencing the endogenous UNC5A. Moreover, colony formation assay indicated that reexpression of UNC5A inhibited the survival of 5637 cells. Together, these data suggest an important role for UNC5A, a candidate tumor suppressor, in predicting response to DNA damage induced by chemotherapeutic drug and regulating cell death in bladder cancer.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1010-4283
1423-0380
DOI:10.1007/s13277-014-1930-0