Effects of multiple doses of ionizing radiation on cytokine expression in rat and human cells

• Published in: International journal of radiation biology Vol. 79; no. 11; pp. 889 - 896
• Main Authors: Boerma, M., Schutte-Bart, C. I., Wedekind, L. E., Beekhuizen, H., Wondergem, J.
• Format: Journal Article
• Language: English
• Published: London Informa UK Ltd 01.11.2003; Taylor & Francis
• Subjects: Animals; Biological and medical sciences; Cell-Free System; Cells, Cultured; cytokines; Cytokines - biosynthesis; DNA, Complementary - metabolism; Dose Fractionation; Dose-Response Relationship, Radiation; Endothelial Cells - radiation effects; Endothelium, Vascular - radiation effects; Enzyme-Linked Immunosorbent Assay; Fibroblast Growth Factor 2 - metabolism; Fibroblasts - metabolism; Humans; Interleukin-8 - metabolism; Medical sciences; Muscle Cells - radiation effects; Platelet-Derived Growth Factor - metabolism; Polymerase Chain Reaction; Proto-Oncogene Proteins c-sis - metabolism; Radiation, Ionizing; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Rats; RNA, Messenger - metabolism; Technology. Biomaterials. Equipments. Material. Instrumentation; Time Factors; Transforming Growth Factor beta - metabolism; Transforming Growth Factor beta1; Biomedical engineering
• ISSN: 0955-3002; 1362-3095
• DOI: 10.1080/09553000310001626117

Purpose: To determine the effect of daily fractionated irradiation on the expression of growth factors and cytokines in different cardiac and vascular cell types. Materials and methods: Cell cultures of rat cardiac myocytes, fibroblasts, a rat cardiac microvascular endothelial cell line and human artery endothelial cells were irradiated with doses of 2 Gy, given daily during 5 consecutive days. Twenty-four hours after each fraction, gene expression was determined by competitive or semiquantitative polymerase chain reaction. Protein secretion into culture media was determined by enzyme-linked immunoabsorbant assay. Results: Of all investigated mRNA levels, transforming growth factor (TGF)-ß1 and fibroblast growth factor (FGF)-2 were slightly upregulated in the rat cardiac endothelial cell line after irradiation. TGF-ß1 protein secretion by these cells was slightly, but non-significantly, elevated. Interleukin 1ß protein levels in myocyte culture media were decreased in control cultures at days 3 and 4 compared with day 2. No significant changes were observed in expression of FGF-2 in either of the four cell types. Moreover, no changes were observed in gene expression of platelet-derived growth factors A, B and interleukin 8 in the human artery endothelial cells. Conclusions: Fractionated irradiation leads to minor changes in the expression of specific cytokines in cardiac myocytes, fibroblasts and endothelial cells.