Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures

• Published in: Applied biochemistry and biotechnology Vol. 180; no. 4; pp. 766 - 779
• Main Authors: Rao, Kiranmayee, Chodisetti, Bhuvaneswari, Gandi, Suryakala, Giri, Archana, Kishor, P. B. Kavi
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2016; Springer Nature B.V
• Subjects: Abutilon indicum; Biochemistry; Biotechnology; callus; Chemistry; Chemistry and Materials Science; Chromatography, High Pressure Liquid; Coumarins - analysis; Cytokinins - pharmacology; Developmental stages; drugs; Embryology; Embryonic growth stage; Embryos; explants; Flowers & plants; Herbal medicine; high performance liquid chromatography; kinetin; Liquid chromatography; Malvaceae - chemistry; manufacturing; Phytochemicals; Plant Somatic Embryogenesis Techniques; plasmolysis; Regeneration - drug effects; scopoletin; Scopoletin - analysis; Seeds - drug effects; shoots; somatic embryogenesis; somatic embryos; Tissue Culture Techniques - methods; Coumarins; Scopoletin; HPLC; Scoparone; Somatic embryogenesis; A. indicum

Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned...