Regeneration-Based Quantification of Coumarins (Scopoletin and Scoparone) in Abutilon indicum In Vitro Cultures

• Published in: Applied biochemistry and biotechnology Vol. 180; no. 4; pp. 766 - 779
• Main Authors: Rao, Kiranmayee, Chodisetti, Bhuvaneswari, Gandi, Suryakala, Giri, Archana, Kishor, P. B. Kavi
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2016; Springer Nature B.V
• Subjects: Abutilon indicum; Biochemistry; Biotechnology; callus; Chemistry; Chemistry and Materials Science; Chromatography, High Pressure Liquid; Coumarins - analysis; Cytokinins - pharmacology; Developmental stages; drugs; Embryology; Embryonic growth stage; Embryos; explants; Flowers & plants; Herbal medicine; high performance liquid chromatography; kinetin; Liquid chromatography; Malvaceae - chemistry; manufacturing; Phytochemicals; Plant Somatic Embryogenesis Techniques; plasmolysis; Regeneration - drug effects; scopoletin; Scopoletin - analysis; Seeds - drug effects; shoots; somatic embryogenesis; somatic embryos; Tissue Culture Techniques - methods; Coumarins; Scopoletin; HPLC; Scoparone; Somatic embryogenesis; A. indicum
• ISSN: 0273-2289; 1559-0291; 1559-0291
• DOI: 10.1007/s12010-016-2131-7

Abutilon indicum exploited for its immense value has been propagated successfully through multiple shoot induction and somatic embryogenesis. Direct regeneration (8.20 ± 0.83 shoots) was achieved from nodal explants using 0.5 mg/l kinetin (Kn) in MS media. The basal callus from nodal explants turned embryogenic on subsequent introduction of 0.2 mg/l TDZ into the Kn-supplemented media, giving rise to somatic embryos. The embryogenic potential of calli expressed in terms of embryo-forming capacity (EFC) increased from 8.15 EFC to 20.95 EFC after plasmolysis. The phytochemical analysis (HPLC) for the presence of scopoletin and scoparone has revealed a unique accumulation pattern, with higher levels of scopoletin during the earlier stages and scoparone in the later stages of development. The embryogenic calli contained the highest amount of coumarins (99.20 ± 0.97 and 61.03 ± 0.47 μg/gFW, respectively) followed by regenerated plant (9.43 ± 0.20 and 36.36 ± 1.19 μg/gFW, respectively), obtained via somatic embryogenesis. Rapid multiplication of A. indicum equipped with two potent coumarins is important in order to meet the commercial demand for combat against dreadful diseases, thereby providing a new platform for plant-based drugs and their manufacture on a commercial scale.