Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8

• Published in: Biofouling (Chur, Switzerland) Vol. 34; no. 2; pp. 132 - 148
• Main Authors: Favre, Laurie, Ortalo-Magné, Annick, Pichereaux, Carole, Gargaros, Audrey, Burlet-Schiltz, Odile, Cotelle, Valérie, Culioli, Gérald
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 07.02.2018; Taylor & Francis Ltd
• Subjects: Analytical chemistry; Arginine; Bacteria; Bacterial Adhesion; Biochemical composition; Biochemistry; Biochemistry, Molecular Biology; Biofilms; Biofouling; Biosynthesis; Cell culture; Cell division; Chemical Sciences; Chromatography, Liquid; Cultures; Genomics; Heme; Histidine; Life Sciences; Lipid composition; Lipids; Liquid chromatography; liquid chromatography-mass spectrometry; Mass spectrometry; Mass spectroscopy; membrane lipids; Membrane proteins; Metabolome; Metabolomics; Mineral nutrients; Nutrient uptake; Ornithine; Peptidases; Phenotype; Phenotypes; Phosphatidylethanolamine; Plankton; Proteins; Proteome; Proteomics; Pseudoalteromonas; Pseudoalteromonas lipolytica TC8; Tandem Mass Spectrometry; Transcription; Transcription factors; Pseudoalteromonas lipolytica TC8; membrane lipids; proteomics; liquid chromatography-mass spectrometry; metabolomics; biofilms; Metabolomics; Membrane lipids; Biofilms; Liquid chromatography-mass spectrometry; Proteomics
• ISSN: 0892-7014; 1029-2454
• DOI: 10.1080/08927014.2017.1413551

A number of bacteria adopt various lifestyles such as planktonic free-living or sessile biofilm stages. This enables their survival and development in a wide range of contrasting environments. With the aim of highlighting specific metabolic shifts between these phenotypes and to improve the overall understanding of marine bacterial adhesion, a dual metabolomics/proteomics approach was applied to planktonic and biofilm cultures of the marine bacterium Pseudoalteromonas lipolytica TC8. The liquid chromatography mass spectrometry (LC-MS) based metabolomics study indicated that membrane lipid composition was highly affected by the culture mode: phosphatidylethanolamine (PEs) derivatives were over-produced in sessile cultures while ornithine lipids (OLs) were more specifically synthesized in planktonic samples. In parallel, differences between proteomes revealed that peptidases, oxidases, transcription factors, membrane proteins and the enzymes involved in histidine biosynthesis were over-expressed in biofilms while proteins involved in heme production, nutrient assimilation, cell division and arginine/ornithine biosynthesis were specifically up-regulated in free-living cells.