Evaluation of Antimicrobial Activity in the Extract of Defatted Hermetia illucens Fed Organic Waste Feed Containing Fermented Effective Microorganisms

• Published in: Animals (Basel) Vol. 12; no. 6; p. 680
• Main Authors: Lee, Kyu-Shik, Yun, Eun-Young, Goo, Tae-Won
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.03.2022; MDPI
• Subjects: Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; antimicrobial peptide; Bacteria; Bacterial infections; Biodiesel fuels; Biofuels; Crushing; Cytotoxicity; Diesel; ecofriendly feedstock; Evaluation; Fatty acids; Feed additives; Feed industry; Feeds; Fermented food; Fertilizers; Food additives; Food industry; Food waste; Gene expression; Hermetia illucens; Hermetia illucens larvae; Insects; Investigations; Larvae; Lipids; Livestock; Livestock feed; Livestock feeds; Microorganisms; Minimum inhibitory concentration; natural antimicrobial substance; Oil; Organic wastes; Pathogens; Pest resistance; Powder; Preservatives; Raw materials; Rural development; Salmonella; Statistical analysis; ecofriendly feedstock; food additives; natural antimicrobial substance; Hermetia illucens larvae; antimicrobial peptide
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani12060680

(black soldier fly) larvae (HIL) are considered useful industrial insects for the production of feed for livestock, eco-friendly fertilizer from organic wastes, and biodiesel. Therefore, we evaluated the antimicrobial activity in the extract of crude-oil-extracted crushed HIL powder prepared from HIL fed organic waste containing fermented effective micro-organisms for biodiesel production. The result showed that antimicrobial activity was not fully induced in HIL fed -containing feed. In contrast, increased antimicrobial activity was observed in defatted HIL extract prepared from crude-oil-extracted crushed HIL powder. We found that the extract effectively inhibited the growth of pathogens and antimicrobial-peptide-resistant bacteria, such as three kinds of species, and , , , , and , with a minimum inhibitory concentration of 200-1000 µg/100 µL. Furthermore, no cytotoxicity to CaCO-2 human intestinal cells was observed in the extract. We also found that the production fee of extract equivalent to the antimicrobial activity of melittin was approximately 25-fold less than the production fee of melittin. Therefore, the results demonstrate that crude-oil-extracted crushed HIL powder prepared from HIL fed organic waste containing fermented effective micro-organisms for biodiesel production should be used as the feedstock for synthetic, preservative-free livestock feed and food additives. Taken together, the present study supports the usefulness of HIL as an eco-friendly feedstock in the biodiesel, agricultural, food, and feed industries.