NF-κB contributes to the detrimental effects of social isolation after experimental stroke

• Published in: Acta neuropathologica Vol. 124; no. 3; pp. 425 - 438
• Main Authors: Venna, Venugopal Reddy, Weston, Gillian, Benashski, Sharon E., Tarabishy, Sami, Liu, Fudong, Li, Jun, Conti, Lisa H., McCullough, Louise D.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.09.2012
• Subjects: Animals; Behavior, Animal - drug effects; Behavior, Animal - physiology; Brain - drug effects; Brain - metabolism; Brain - pathology; Brain - physiopathology; Brain Ischemia - metabolism; Brain Ischemia - pathology; Brain Ischemia - physiopathology; Cerebral blood flow; Female; Genetic engineering; Injuries; Interleukin 6; Interleukin-6 - blood; Ischemia; Male; Medicine; Medicine & Public Health; Mice; Mice, Knockout; Motor Activity - drug effects; Motor Activity - physiology; Neurons - drug effects; Neurons - metabolism; Neurons - pathology; Neurosciences; NF- Kappa B protein; NF-kappa B - antagonists & inhibitors; NF-kappa B - genetics; NF-kappa B - metabolism; Nuclear transport; Original Paper; Ovariectomy; Pathology; Pyrrolidines - pharmacology; Recovery of function; Recovery of Function - drug effects; Recovery of Function - physiology; Risk factors; Signal Transduction - drug effects; Social interactions; Social Isolation; Stroke; Stroke - metabolism; Stroke - pathology; Stroke - physiopathology; Thiocarbamates - pharmacology; Transcription; Western blotting; Isolation; Neuroinflammation; NF-kappaB; Pair housing; Middle cerebral artery occlusion
• ISSN: 0001-6322; 1432-0533
• DOI: 10.1007/s00401-012-0990-8

Social isolation (SI) is increasingly recognized as a risk factor for stroke. Individuals with lack of social support systems have an increased incidence of stroke, poorer recovery, and greater functional decline after injury compared to individuals with social support. Attesting to the importance of social factors in stroke outcome is that these same effects can be reproducibly demonstrated in animals; social interaction improves behavioral deficits and reduces damage after experimental stroke, whereas SI enhances injury. The mechanism by which SI exacerbates injury is unclear. We investigated the role of nuclear factor-kappaB (NF-κB) signaling in male mice that were pair housed (PH) with an ovariectomized female prior to random assignment into continued PH or SI for 7 days prior to middle cerebral artery occlusion. The effects of SI on infarct volume and functional recovery were assessed at 72 h post-stroke. Nuclear NF-κB levels and activity were assessed by Western blot and transcriptional assays. SI significantly exacerbated infarct size in both male and female mice compared to PH mice. SI mice had delayed functional recovery compared to PH mice. An elevation of systemic IL-6 levels, increased nuclear NF-κB transcriptional activity, and enhanced nuclear translocation of NF-κB was seen in SI stroke animals. Interference with NF-κB signaling using either a pharmacological inhibitor or genetically engineered NF-κB p50 knockout mice abolished the detrimental effects of SI on both infarct size and functional recovery. This suggests that NF-κB mediates the detrimental effects of SI.