The Comparison of the Effect of Endodontic Irrigation on Cell Adherence to Root Canal Dentin

• Published in: Journal of endodontics Vol. 34; no. 12; pp. 1474 - 1479
• Main Authors: Ring, Karla C., DMD, Murray, Peter E., PhD, Namerow, Kenneth N., DDS, Kuttler, Sergio, DDS, Garcia-Godoy, Franklin, DDS, MS
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.12.2008
• Subjects: Biocompatibility; Biocompatible Materials - pharmacology; Biocompatible Materials - toxicity; Cell Adhesion - drug effects; Cell Line; Cell Shape - drug effects; Chelating Agents - pharmacology; Chelating Agents - toxicity; Chlorhexidine - analogs & derivatives; Chlorhexidine - pharmacology; Chlorhexidine - toxicity; Citric Acid - pharmacology; Citric Acid - toxicity; Dental Pulp - cytology; Dental Pulp - drug effects; Dental Pulp Cavity - drug effects; Dentistry; Doxycycline - pharmacology; Doxycycline - toxicity; Edetic Acid - pharmacology; Edetic Acid - toxicity; Endocrinology & Metabolism; Fibroblasts - drug effects; Humans; Hydrogen Peroxide - pharmacology; Hydrogen Peroxide - toxicity; Microscopy, Electron, Scanning; Morinda; Plant Extracts - pharmacology; Plant Extracts - toxicity; Polysorbates - pharmacology; Polysorbates - toxicity; regenerative endodontics; Root Canal Irrigants - pharmacology; Root Canal Irrigants - toxicity; Root Canal Preparation - instrumentation; Root Canal Preparation - methods; Smear Layer; Sodium Hypochlorite - pharmacology; Sodium Hypochlorite - toxicity; stem cells; Stem Cells - drug effects; Tissue Culture Techniques; stem cells; Biocompatibility; smear layer; regenerative endodontics
• ISSN: 0099-2399; 1878-3554
• DOI: 10.1016/j.joen.2008.09.001

Abstract The purpose of this study was to compare the effect of 10 different endodontic irrigation and chelating treatments on dental pulp stem cell (DPSC) attachment to root canal surfaces. Thirty-eight extracted human nondiseased single-canal teeth were cleaned and shaped using ProTaper and ProFile rotary instrumentation (Tulsa Dentsply, Tulsa, OK). The irrigation treatments investigated were 6% sodium hypochlorite, 2% chlorhexidine gluconate, Aquatine Endodontic Cleanser, and Morinda citrifolia juice. The irrigation treatments were used in conjunction with EDTA or MTAD. The instrumented teeth were immediately placed in cell culture with confluent DPSCs for 1 week. The number of attached DPSCs appeared to be correlated with the cytotoxicity of the root canal irrigating solution (analysis of variance, p < 0.0001). The presence or absence of the smear layer had little influence on DPSC activity (chi-square, p > 0.05). The results suggest that biocompatible irrigants are needed to promote DPSC attachment to root canal dentin, which is essential to accomplish some regenerative endodontic therapies.