Hydrogel Microparticles as Sensors for Specific Adhesion: Case Studies on Antibody Detection and Soil Release Polymers

• Published in: Gels Vol. 3; no. 3; p. 31
• Main Authors: Strzelczyk, Alexander Klaus, Wang, Hanqing, Lindhorst, Andreas, Waschke, Johannes, Pompe, Tilo, Kropf, Christian, Luneau, Benoit, Schmidt, Stephan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.08.2017; MDPI
• Subjects: Adhesion tests; Adhesives; Antibodies; Antigens; biointerface; Biological activity; biomimetic hydrogel; Cancer; Carbohydrates; contact mechanics; elastic solids; Energy measurement; Enzymes; Hydrogels; Immunoglobulins; Investigations; Methods; Microparticles; Microscopy; poly(ethylene glycol) (PEG); Polymers; Proteins; reflection interference contrast microscopy (RICM); Sensors; soft colloidal probe; Soil investigations; Soil properties; United States > US; Germany; elastic solids; biomimetic hydrogel; soft colloidal probe; contact mechanics; reflection interference contrast microscopy (RICM); biointerface; poly(ethylene glycol) (PEG)
• ISSN: 2310-2861; 2310-2861
• DOI: 10.3390/gels3030031

Adhesive processes in aqueous media play a crucial role in nature and are important for many technological processes. However, direct quantification of adhesion still requires expensive instrumentation while their sample throughput is rather small. Here we present a fast, and easily applicable method on quantifying adhesion energy in water based on interferometric measurement of polymer microgel contact areas with functionalized glass slides and evaluation via the Johnson⁻Kendall⁻Roberts (JKR) model. The advantage of the method is that the microgel matrix can be easily adapted to reconstruct various biological or technological adhesion processes. Here we study the suitability of the new adhesion method with two relevant examples: (1) antibody detection and (2) soil release polymers. The measurement of adhesion energy provides direct insights on the presence of antibodies showing that the method can be generally used for biomolecule detection. As a relevant example of adhesion in technology, the antiadhesive properties of soil release polymers used in today's laundry products are investigated. Here the measurement of adhesion energy provides direct insights into the relation between polymer composition and soil release activity. Overall, the work shows that polymer hydrogel particles can be used as versatile adhesion sensors to investigate a broad range of adhesion processes in aqueous media.