Regulation of mouse ornithine decarboxylase activity by cell growth, serum and tetradecanoyl phorbol acetate is governed primarily by sequences within the coding region of the gene

• Published in: Nucleic acids research Vol. 17; no. 23; pp. 9843 - 9860
• Main Authors: VAN DAALEN WETTERS, T, BRABANT, M, COFFINO, P
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 11.12.1989
• Subjects: Animals; Biological and medical sciences; Cell Division; Cell Line; Cells, Cultured; Culture Media; DNA, Recombinant - metabolism; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Enzymologic - drug effects; Genes - drug effects; Kinetics; Lymphoma; Mice; Molecular and cellular biology; Molecular genetics; Ornithine Decarboxylase - biosynthesis; Ornithine Decarboxylase - genetics; Ornithine Decarboxylase - metabolism; Tetradecanoylphorbol Acetate - pharmacology; Transfection; Cell proliferation; Rodentia; 3T3-Cell line; Gene organization; Ornithine decarboxylase; Recombinant DNA; Flanking sequence; Gene expression; Vertebrata; Regulation(control); Mammalia; Messenger RNA; Transfection; Enzymatic activity; Mouse; Serum
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/17.23.9843

To determine the genetic elements required for modulation of ornithine decarboxylase (ODC) activity in response to cell growth or treatment with serum or with tetradecanoyl phorbol acetate, ODC-deficient cells were transfected with a series of recombinant DNAs encoding mouse ODC. All of the transfected cells expressing an intact mouse ODC protein displayed regulation of ODC activity, including those expressing a construct deprived of all ODC-specific sequence information except the protein-coding region. ODC mRNA changed much less than enzymatic activity. A mutation of the protein-coding region that converted ODC from an unstable to a stable intracellular protein attenuated the regulatory response. We conclude that post-transcriptional events associated with ODC degradation dominate the response to these stimuli.