Incidence of hemoparasitic infections in cattle from central and northern Thailand

• Published in: PeerJ (San Francisco, CA) Vol. 10; p. e13835
• Main Authors: Koonyosying, Pongpisid, Rittipornlertrak, Amarin, Chomjit, Paweena, Sangkakam, Kanokwan, Muenthaisong, Anucha, Nambooppha, Boondarika, Srisawat, Wanwisa, Apinda, Nisachon, Singhla, Tawatchai, Sthitmatee, Nattawooti
• Format: Journal Article
• Language: English
• Published: United States PeerJ, Inc 10.08.2022; PeerJ Inc
• Subjects: Agricultural Science; Anaplasma spp; Anaplasmosis - epidemiology; Anemia; Animals; Animals, Domestic - genetics; Antigens; Arachnids; Babesia spp; Babesiosis - epidemiology; Beef cattle; Blood; Blood parasites; Cattle; Cattle Diseases - epidemiology; Cell size; DNA sequencing; Epidemiology; Farm management; Genetic diversity; Hematocrit; Hemoparasites; Incidence; Infections; Livestock; Microbiology; Parasites; Parasitic diseases; Parasitology; Phylogeny; Proteins; Provinces; Sequence analysis; Sequence Analysis, DNA - veterinary; Thailand; Thailand - epidemiology; Theileria - genetics; Theileria spp; Theileriasis - epidemiology; Vector-borne diseases; Veterinary Medicine; Zoology; Thailand; United States > US; Hemoparasites; Thailand; Cattle; Anaplasma spp; Babesia spp; Theileria spp
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.13835

Hemoparasites, such as spp., spp. and spp., can negatively affect the health of farm animals resulting in significant losses in production. These losses inherently affect the economics of the livestock industry. Since increases in the severity of vector-borne diseases in the southeast Asian region have been reported, investigations of parasitic epidemiology in Thailand will be necessary to improve the existing parasite control strategies for blood parasitic infections. This study aims to investigate incidences of bovine hemoparasites throughout central and northern Thailand by focusing on areas of high-density cattle populations. Blood parasitic infections among cattle were screened and identified by microscopic examination. Anemia status was then determined by evaluation of the packed cell volume (PCV) of each animal. Furthermore, blood parasites were detected and identified by genus and species-specific primers through the polymerase chain reaction method. Amplicons were subjected to DNA sequencing; thereafter, phylogenetic trees were constructed to determine the genetic diversity and relationships of the parasite in each area. A total of 1,066 blood samples were found to be positive for blood parasitic infections as follows: 13 (1.22%), 389 (36.50%), and 364 (34.15%) for , and , respectively. Furthermore, multiple hemoparasitic infections in the cattle were detected. The hematocrit results revealed 161 hemoparasitic infected samples from 965 blood samples, all of which exhibiting indications of anemia with no significant differences. Sequence analysis of the identified isolates in this study revealed that , four separate clades of , and exhibited considerable sequence similarity to homologous sequences from isolates obtained from other countries. Sequence similarity ranged between 98.57-100%, 83.96-100%, and 97.60-100% for , , and , respectively. In this study, the analyzed incidence data of cattle hemoparasitic infection in Thailand has provided valuable and basic information for the adaptation of blood-borne parasitic infections control strategies. Moreover, the data obtained from this study would be useful for future effective parasitic disease prevention and surveillance among cattle.