Codon changed immobilization antigen (iAg), a potent DNA vaccine in fish against Cryptocaryon irritans infection

• Published in: Vaccine Vol. 30; no. 5; pp. 893 - 903
• Main Authors: Jose Priya, T.A, Lin, Yen-Hong, Wang, Yu-Chi, Yang, Chii-Shen, Chang, Poh-Shing, Song, Yen-Ling
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 20.01.2012
• Subjects: Allergy and Immunology; amino acids; Animals; antigens; Antigens, Protozoan - genetics; Antigens, Protozoan - immunology; antiserum; Ciliophora - genetics; Ciliophora - immunology; Ciliophora Infections - immunology; Ciliophora Infections - prevention & control; Ciliophora Infections - veterinary; Codon change; Cryptocaryon irritans; DNA vaccine; DNA, Protozoan - chemistry; DNA, Protozoan - genetics; emulsions; enzyme-linked immunosorbent assay; eukaryotic cells; Fish Diseases - immunology; Fish Diseases - prevention & control; grouper; Immobilization antigen; immunization; Injections, Intramuscular; intramuscular injection; Marine; Molecular Sequence Data; plasmids; Protozoa; Protozoan Infections, Animal - immunology; Protozoan Infections, Animal - prevention & control; Protozoan Proteins - genetics; Protozoan Proteins - immunology; Protozoan Vaccines - administration & dosage; Protozoan Vaccines - genetics; Protozoan Vaccines - immunology; recombinant vaccines; Sequence Analysis, DNA; stop codon; Survival Analysis; Vaccines, DNA - administration & dosage; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Western blotting; DNA vaccine; Codon change; Cryptocaryon irritans; Immobilization antigen
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2011.11.102

Highlights ► Codon changed iAg (optiAg) expressed in both prokaryotic and eukaryotic cell systems. ► In vitro immunological data confirmed that optiAg retained the native confirmation. ► iAg is immunogenic due to the protection it conferred to the immunized fish. ► iAg can be a potent DNA vaccine in fish against infection by C. irritans.