Comparison between three adjuvants for a vaccine against canine leishmaniasis: In vitro evaluation of macrophage killing ability

• Published in: Comparative immunology, microbiology and infectious diseases Vol. 33; no. 2; pp. 175 - 182
• Main Authors: Trotta, T, Fasanella, A, Scaltrito, D, Gradoni, L, Mitolo, V, Brandonisio, O, Acquafredda, A, Panaro, M.A
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.03.2010; Elsevier
• Subjects: Adjuvant; Adjuvants, Immunologic - therapeutic use; Allergy and Immunology; Animals; Antigens, Protozoan - immunology; BCG Vaccine - immunology; BCG Vaccine - therapeutic use; Biological and medical sciences; Cell Wall Skeleton - immunology; Cell Wall Skeleton - therapeutic use; Cord Factors - immunology; Cord Factors - therapeutic use; Dog; Dog Diseases - immunology; Dog Diseases - parasitology; Dog Diseases - prevention & control; Dogs; Female; Fundamental and applied biological sciences. Psychology; Humans; Infectious Disease; Leishmania; Leishmania infantum; Leishmania infantum - immunology; Leishmaniasis - immunology; Leishmaniasis - parasitology; Leishmaniasis - prevention & control; Leishmaniasis - veterinary; Leishmaniasis Vaccines - immunology; Leishmaniasis Vaccines - therapeutic use; Leishmaniosis; Leukocytes, Mononuclear - immunology; Lipid A - analogs & derivatives; Lipid A - immunology; Lipid A - therapeutic use; Macrophages - immunology; Male; Protozoa; Time Factors; Vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Adjuvant; Vaccine; Leishmaniosis; Dog; Leishmania; Kinetoplastida; Fissipedia; Protozoa; Carnivora; Protozoal disease; Parasitosis; Leishmaniasis; In vitro; Infection; Vertebrata; Mammalia; Immunology; Immunological adjuvant; Veterinary; Macrophage
• ISSN: 0147-9571; 1878-1667
• DOI: 10.1016/j.cimid.2009.12.003

Abstract The aim of this study was to evaluate, in terms of dog macrophage killing ability in vitro, a vaccine based on Leishmania infantum promastigote soluble antigen (LSA) formulated with three different adjuvants (BCG, AdjuPrime™, MPL/TDM/CWS). A significant increase of the macrophage killing ability was observed in dogs vaccinated with LSA + MPL/TDM/CWS after 1 month from vaccination. A similar increase of macrophage parasitocidal ability was present only after 5 months in dogs vaccinated with LSA + BCG or LSA + AdjuPrime™. In all dogs the augmented killing percentage was still present after 12 months from vaccination. Therefore, in particular LSA + MPL/TDM/CWS vaccine seems promising for further studies in dogs.