A fluorescent DNA probe prepared by the direct derivatization of the sugar moiety for hybridization assay

The preparation of a fluorescent DNA probe based on the derivatization of the terminal hydroxyl group of the sugar moiety of a DNA primer and its applicability to the DNA hybridization assay are described. M13mp8 plasmid primer reacts with 2-(5-chlorocarbonyl-2-oxazolyl)-5,6-methylenedioxybenzofu ra...

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Bibliographic Details
Published inChemical & pharmaceutical bulletin Vol. 40; no. 10; pp. 2852 - 2853
Main Authors NAGAOKA, H, NOHTA, H, SAITO, M, OHKURA, Y
Format Journal Article
LanguageEnglish
Published Tokyo Maruzen 1992
Subjects
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Carbohydrates
DNA Probes
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Molecular Sequence Data
Nucleic Acid Hybridization
Nucleic acids
Molecular hybridization
Fluorescent tracer
Chemical reaction
DNA
Preparation
Molecular probe
Derivatization
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Summary:The preparation of a fluorescent DNA probe based on the derivatization of the terminal hydroxyl group of the sugar moiety of a DNA primer and its applicability to the DNA hybridization assay are described. M13mp8 plasmid primer reacts with 2-(5-chlorocarbonyl-2-oxazolyl)-5,6-methylenedioxybenzofu ran in the presence of sodium azide to form the corresponding fluorescent probe, which can be used for the hybridization assay to the target DNA, M13mp8 plasmid vector. The detection limit of the DNA with the naked eye is 10 ng (approximately 300 fmol)/spot on filters for the hybridization assay.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.40.2852