Genomics and cellulolytic, hemicellulolytic, and amylolytic potential of Iocasia fonsfrigidae strain SP3-1 for polysaccharide degradation

• Published in: PeerJ (San Francisco, CA) Vol. 10; p. e14211
• Main Authors: Heng, Sobroney, Sutheeworapong, Sawannee, Champreda, Verawat, Uke, Ayaka, Kosugi, Akihiko, Pason, Patthra, Waeonukul, Rattiya, Ceballos, Ruben Michael, Ratanakhanokchai, Khanok, Tachaapaikoon, Chakrit
• Format: Journal Article
• Language: English
• Published: United States PeerJ, Inc 19.10.2022; PeerJ Inc
• Subjects: Acetic acid; Alginic acid; Amylolytic enzyme; Arabinose; Bacteria; Bacteria, Anaerobic; Biodiversity; Biomass; Biotechnology; Butanol; Carbohydrate-binding module; Carbon dioxide; Cell culture; Cellobiose; Cellulolytic enzyme; Cellulose; D-Galactose; D-Rhamnose; DNA; Drug tolerance; Electron microscopy; Enzymes; Fatty acids; Firmicutes - genetics; Fructose; Galactose; Genes; Genomes; Genomics; Glucose; Glycerol; Halophilic alkaliphilic anaerobic bacterium; Hemicellulolytic enzyme; Hybridization; Identification systems; Iocasia fonsfrigidae; L-Arabinose; Lactose; Lignin; Lipid composition; Metabolism; Microbiology; Microorganisms; Molecular Biology; Nucleotide sequence; pH effects; Phylogenetics; Phylogeny; Physiology; Polysaccharides; Proteins; RNA, Ribosomal, 16S - genetics; Salinity tolerance; Sequence Analysis, DNA; Sodium Chloride; Sugars; Taxonomy; Thailand; Japan; Hemicellulolytic enzyme; Carbohydrate-binding module; Halophilic alkaliphilic anaerobic bacterium; Iocasia fonsfrigidae; Cellulolytic enzyme; Amylolytic enzyme
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.14211

Cellulolytic, hemicellulolytic, and amylolytic (CHA) enzyme-producing halophiles are understudied. The recently defined taxon consists of one well-described anaerobic bacterial strain: NS-1 . Prior to characterization of strain NS-1 , an isolate designated sp. SP3-1 was isolated and its genome was published. Based on physiological and genetic comparisons, it was suggested that sp. SP3-1 may be another isolate of . Despite being geographic variants of the same species, data indicate that strain SP3-1 exhibits genetic, genomic, and physiological characteristics that distinguish it from strain NS-1 . In this study, we examine the halophilic and alkaliphilic nature of strain SP3-1 and the genetic substrates underlying phenotypic differences between strains SP3-1 and NS-1 with focus on sugar metabolism and CHA enzyme expression. Standard methods in anaerobic cell culture were used to grow strains SP3-1 as well as other comparator species. Morphological characterization was done via electron microscopy and Schaeffer-Fulton staining. Data for sequence comparisons ( , 16S rRNA) were retrieved via BLAST and EzBioCloud. Alignments and phylogenetic trees were generated via CLUTAL_X and neighbor joining functions in MEGA (version 11). Genomes were assembled/annotated via the Prokka annotation pipeline. Clusters of Orthologous Groups (COGs) were defined by eegNOG 4.5. DNA-DNA hybridization calculations were performed by the ANI Calculator web service. Cells of strain SP3-1 are rods. SP3-1 cells grow at NaCl concentrations of 5-30% (w/v). Optimal growth occurs at 37 °C, pH 8.0, and 20% NaCl (w/v). Although phylogenetic analysis based on 16S rRNA gene indicates that strain SP3-1 belongs to the genus with 99.58% average nucleotide sequence identity to NS-1 , strain SP3-1 is uniquely an extreme haloalkaliphile. Moreover, strain SP3-1 ferments D-glucose to acetate, butyrate, carbon dioxide, hydrogen, ethanol, and butanol and will grow on L-arabinose, D-fructose, D-galactose, D-glucose, D-mannose, D-raffinose, D-xylose, cellobiose, lactose, maltose, sucrose, starch, xylan and phosphoric acid swollen cellulose (PASC). D-rhamnose, alginate, and lignin do not serve as suitable culture substrates for strain SP3-1. Thus, the carbon utilization profile of strain SP3-1 differs from that of strain NS-1 . Differences between these two strains are also noted in their lipid composition. Genomic data reveal key differences between the genetic profiles of strain SP3-1 and NS-1 that likely account for differences in morphology, sugar metabolism, and CHA-enzyme potential. Important to this study, SP3-1 produces and extracellularly secretes CHA enzymes at different levels and composition than type strain NS-1 . The high salt tolerance and pH range of SP3-1 makes it an ideal candidate for salt and pH tolerant enzyme discovery.