Long noncoding RNA HOTAIR is a prognostic biomarker and inhibits chemosensitivity to doxorubicin in bladder transitional cell carcinoma

• Published in: Cancer chemotherapy and pharmacology Vol. 77; no. 3; pp. 507 - 513
• Main Authors: Shang, Chao, Guo, Yan, Zhang, Hui, Xue, Yi-xue
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2016; Springer Nature B.V
• Subjects: Aged; Antibiotics, Antineoplastic - pharmacology; Apoptosis - drug effects; Biomarkers, Tumor - genetics; Cancer Research; Carcinoma, Transitional Cell - drug therapy; Carcinoma, Transitional Cell - genetics; Carcinoma, Transitional Cell - pathology; Cell Proliferation - drug effects; Doxorubicin - pharmacology; Female; Flow Cytometry; Gene Expression Regulation, Neoplastic - genetics; Humans; Kaplan-Meier Estimate; Male; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Article; Pharmacology/Toxicology; Prognosis; RNA, Long Noncoding - genetics; Survival Rate; Up-Regulation - genetics; Urinary Bladder Neoplasms - drug therapy; Urinary Bladder Neoplasms - genetics; Urinary Bladder Neoplasms - pathology; HOTAIR; Chemotherapy; Bladder transitional cell carcinoma; Long noncoding RNA; Doxorubicin
• ISSN: 0344-5704; 1432-0843
• DOI: 10.1007/s00280-016-2964-3

Purpose In this study, whether HOTAIR is a prognostic biomarker will be detected, and its regulative effects of chemosensitivity to doxorubicin in TCC cells will be examined. Methods The expression of HOTAIR was detected by quantitative real-time PCR. Overall survival rate was calculated by Kaplan–Meier method with the log-rank test for comparisons. MTT assay was used to detect cell proliferation ability and chemosensitivity. Dual-color flow cytometric method was used to detect cell apoptosis. Results HOTAIR was up-regulated in bladder transitional cell carcinoma (TCC) tissues and cell lines compared with normal bladder transitional cell (NBTC) tissues and bladder epithelial immortalized SV-HUC-1 cells, and its expression level had positive correlation with histological grades of TCC. Moreover, HOTAIR was an independent prognostic biomarker of overall survival for TCC patients. The expression and silence vector for HOTAIR were transfected into T24 and J82 cells to up-regulate and silence the HOTAIR expression, respectively. In T24 and J82 cells, HOTAIR over-expression promoted cell proliferation and inhibited chemosensitivity to doxorubicin and cell apoptosis induced by doxorubicin; silence of HOTAIR showed opposite regulative effects. Conclusions In summary, lncRNA HOTAIR was an independent prognostic biomarker of overall survival in TCC patients and could regulate chemosensitivity to doxorubicin of human TCC cells. HOTAIR might provide a new potential therapeutic target and stratagem for TCC.