Identification of HMMR as a prognostic biomarker for patients with lung adenocarcinoma via integrated bioinformatics analysis

• Published in: PeerJ (San Francisco, CA) Vol. 9; p. e12624
• Main Authors: Li, Zhaodong, Fei, Hongtian, Lei, Siyu, Hao, Fengtong, Yang, Lijie, Li, Wanze, Zhang, Laney, Fei, Rui
• Format: Journal Article
• Language: English
• Published: United States PeerJ, Inc 22.12.2021; PeerJ Inc
• Subjects: Adenocarcinoma; Bioinformatics; Biomarkers; Cell Biology; Cell cycle; Connectivity; Correlation analysis; Datasets; Differentially expressed genes; Gene expression; Gene set enrichment analysis; Genomes; Genomics; HMMR; Identification; Kinases; Lung adenocarcinoma; Lung cancer; Lung carcinoma; Medical Genetics; Medical prognosis; Meta-analysis; Oncology; Patients; Prognosis; Protein interaction; Proteins; Respiratory Medicine; Signal transduction; Software; Statistical analysis; Survival analysis; Therapeutic targets; Tumors; Differentially expressed genes; Prognosis; HMMR; Lung adenocarcinoma; Bioinformatics
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.12624

Lung adenocarcinoma (LUAD) is the most prevalent tumor in lung carcinoma cases and threatens human life seriously worldwide. Here we attempt to identify a prognostic biomarker and potential therapeutic target for LUAD patients. Differentially expressed genes (DEGs) shared by GSE18842, GSE75037, GSE101929 and GSE19188 profiles were determined and used for protein-protein interaction analysis, enrichment analysis and clinical correlation analysis to search for the core gene, whose expression was further validated in multiple databases and LUAD cells (A549 and PC-9) by quantitative real-time PCR (qRT-PCR) and western blot analyses. Its prognostic value was estimated using the Kaplan-Meier method, meta-analysis and Cox regression analysis based on the Cancer Genome Atlas (TCGA) dataset and co-expression analysis was conducted using the Oncomine database. Gene Set Enrichment Analysis (GSEA) was performed to illuminate the potential functions of the core gene. A total of 115 shared DEGs were found, of which 24 DEGs were identified as candidate hub genes with potential functions associated with cell cycle and transcription factor network. Among these candidates, was identified as the core gene, which was highly expressed in LUAD as verified by multiple datasets and cell samples. Besides, high expression was found to independently predict poor survival in patients with LUAD. Co-expression analysis showed that was closely related to and was mainly involved in cell cycle as suggested by GSEA. might be served as an independent prognostic biomarker for LUAD patients, which needs further validation in subsequent studies.