A Potential Anthelmintic Phytopharmacological Source of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep

• Published in: Animals (Basel) Vol. 13; no. 1; p. 45
• Main Authors: Štrbac, Filip, Krnjajić, Slobodan, Maurelli, Maria Paola, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Knežević, Goran, Cringoli, Giuseppe, Rinaldi, Laura, Bosco, Antonio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.12.2022; MDPI
• Subjects: Animals; Anthelmintic agents; anthelmintic resistance; Antiparasitic agents; Biocompatibility; Blood; Carvacrol; Drug development; Drugs; Economic impact; Eggs; Essential oils; Evaluation; Feces; Flowers & plants; gastrointestinal nematodes; Hatchability; In vitro methods and tests; in vitro test; In vivo methods and tests; in vivo test; Intestinal parasites; Kidneys; Liver; Nematodes; Oils & fats; Oral administration; Oregano; Origanum vulgare; Parasites; Pest resistance; Sheep; Side effects; Toxicity; Toxicity testing; Veterinary medicine; Serbia; Italy; Europe; gastrointestinal nematodes; in vitro test; in vivo test; essential oils; anthelmintic resistance; oregano
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani13010045

The development of anthelmintic resistance in sheep gastrointestinal nematodes (GINs) requires novel strategies for the sustainable control of these parasites. This study aimed to evaluate the anthelmintic efficacy of the (L.) essential oil (EO) against sheep GINs and to evaluate the possibility of its use in control practice. The in vitro egg hatch test was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025, and 0.0125 mg/mL) of the tested EO. For the in vivo fecal egg count reduction test, the EO of was administrated orally at a mean single dose of 150 mg/kg to sheep from two farms in Southern Italy, whereby potential toxic effects to the hosts were also evaluated. In the egg hatch test, the inhibition of egg hatchability varied from 71.3% to 93.7%, depending on the concentration used. The high anthelmintic potential was confirmed in the fecal egg count reduction test with an average reduction of nematode eggs in feces of 43.2% and 60.1% on days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during the clinical examination of sheep or by observing blood count and liver or kidney function test results. The obtained results suggest the strong activity of the EO against sheep GINs, probably due to a high percentage of carvacrol (76.21%), whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, it is suitable for use in veterinary practice as a part of an integrated strategy for the control of sheep GINs.