Significant variations in alternative splicing patterns and expression profiles between human-mouse orthologs in early embryos

Human and mouse orthologs are expected to have similar biological functions; however, many discrepancies have also been reported. We systematically compared human and mouse orthologs in terms of alternative splicing patterns and expression profiles. Human-mouse orthologs are divergent in alternative...

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Published inScience China. Life sciences Vol. 60; no. 2; pp. 178 - 188
Main Authors Chen, Geng, Chen, Jiwei, Yang, Jianmin, Chen, Long, Qu, Xiongfei, Shi, Caiping, Ning, Baitang, Shi, Leming, Tong, Weida, Zhao, Yongxiang, Zhang, Meixia, Shi, Tieliu
Format Journal Article
LanguageEnglish
Published Beijing Science China Press 01.02.2017
Springer Nature B.V
Subjects
Alternative Splicing
Animals
Biomedical and Life Sciences
Embryonic Development - genetics
Exons
Gene Expression Regulation, Developmental
Humans
Life Sciences
Mice
Protein Isoforms - genetics
Research Paper
Sequence Analysis, RNA
Single-Cell Analysis
Species Specificity
Transcription Factors - genetics
Transcriptome
同源基因
小鼠
早期胚胎
生物学功能
胚胎发育过程
胚胎发育阶段
表达谱
选择性剪接
ortholog
alternative splicing
RNA-seq
early embryo
gene expression
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Summary:Human and mouse orthologs are expected to have similar biological functions; however, many discrepancies have also been reported. We systematically compared human and mouse orthologs in terms of alternative splicing patterns and expression profiles. Human-mouse orthologs are divergent in alternative splicing, as human orthologs could generally encode more isoforms than their mouse orthologs. In early embryos, exon skipping is far more common with human orthologs, whereas constitutive exons are more prevalent with mouse orthologs. This may correlate with divergence in expression of splicing reg- ulators. Orthologous expression similarities are different in distinct embryonic stages, with the highest in morula. Expression differences for orthologous transcription factor genes could play an important role in orthologous expression discordance. We further detected largely orthologous divergence in differential expression between distinct embryonic stages. Collectively, our study uncovers significant orthologous divergence from multiple aspects, which may result in functional differences and dy- namics between human-mouse orthologs during embryonic development.
Bibliography:Human and mouse orthologs are expected to have similar biological functions; however, many discrepancies have also been reported. We systematically compared human and mouse orthologs in terms of alternative splicing patterns and expression profiles. Human-mouse orthologs are divergent in alternative splicing, as human orthologs could generally encode more isoforms than their mouse orthologs. In early embryos, exon skipping is far more common with human orthologs, whereas constitutive exons are more prevalent with mouse orthologs. This may correlate with divergence in expression of splicing reg- ulators. Orthologous expression similarities are different in distinct embryonic stages, with the highest in morula. Expression differences for orthologous transcription factor genes could play an important role in orthologous expression discordance. We further detected largely orthologous divergence in differential expression between distinct embryonic stages. Collectively, our study uncovers significant orthologous divergence from multiple aspects, which may result in functional differences and dy- namics between human-mouse orthologs during embryonic development.
ortholog, alternative splicing, RNA-seq, early embryo, gene expression
11-5841/Q
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1674-7305
1869-1889
DOI:10.1007/s11427-015-0348-5