Enhanced Ca2+ influx in mechanically distorted erythrocytes measured with 19F nuclear magnetic resonance spectroscopy

• Published in: Scientific reports Vol. 11; no. 1; p. 3749
• Main Authors: Kuchel, Philip W., Romanenko, Konstantin, Shishmarev, Dmitry, Galvosas, Petrik, Cox, Charles D.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 12.02.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 631/57; 631/80; Acids; Adenosine triphosphate; Blood; Calcimycin; Calcium influx; Cell suspensions; Compression; Erythrocytes; Ethane; Gelatin; Glucose; Glycolysis; Humanities and Social Sciences; Magnetic resonance spectroscopy; multidisciplinary; NMR; Nuclear magnetic resonance; Proteins; Pyrazine; Science; Science (multidisciplinary); Signal to noise ratio; Spectroscopy; Spectrum analysis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-021-83044-z

We present the first direct nuclear magnetic resonance (NMR) evidence of enhanced entry of Ca 2+ ions into human erythrocytes (red blood cells; RBCs), when these cells are mechanically distorted. For this we loaded the RBCs with the fluorinated Ca 2+ chelator, 1,2-bis(2-amino-5-fluorophenoxy)ethane- N,N,N′,N′ -tetraacetic acid (5FBAPTA), and recorded 19 F NMR spectra. The RBCs were suspended in gelatin gel in a special stretching/compression apparatus. The 5FBAPTA was loaded into the cells as the tetraacetoxymethyl ester; and 13 C NMR spectroscopy with [1,6- 13 C] d -glucose as substrate showed active glycolysis albeit at a reduced rate in cell suspensions and gels. The enhancement of Ca 2+ influx is concluded to be via the mechanosensitive cation channel Piezo1. The increased rate of influx brought about by the activator of Piezo1, 2-[5-[[(2,6-dichlorophenyl)methyl]thio]-1,3,4-thiadiazol-2-yl]-pyrazine (Yoda1 ) supported this conclusion; while the specificity of the cation-sensing by 5FBAPTA was confirmed by using the Ca 2+ ionophore, A23187.