A three-photon head-mounted microscope for imaging all layers of visual cortex in freely moving mice
Advances in head-mounted microscopes have enabled imaging of neuronal activity using genetic tools in freely moving mice but these microscopes are restricted to recording in minimally lit arenas and imaging upper cortical layers. Here we built a 2-g, three-photon excitation-based microscope, contain...
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Published in | Nature methods Vol. 20; no. 4; pp. 610 - 616 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Nature Publishing Group US
01.04.2023
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Advances in head-mounted microscopes have enabled imaging of neuronal activity using genetic tools in freely moving mice but these microscopes are restricted to recording in minimally lit arenas and imaging upper cortical layers. Here we built a 2-g, three-photon excitation-based microscope, containing a
z
-drive that enabled access to all cortical layers while mice freely behaved in a fully lit environment. The microscope had on-board photon detectors, robust to environmental light, and the arena lighting was timed to the end of each line-scan, enabling functional imaging of activity from cortical layer 4 and layer 6 neurons expressing jGCaMP7f in mice roaming a fully lit or dark arena. By comparing the neuronal activity measured from populations in these layers we show that activity in cortical layer 4 and layer 6 is differentially modulated by lit and dark conditions during free exploration.
A lightweight three-photon miniature microscope allows imaging neuronal activity throughout the cortex in freely moving mice. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1548-7091 1548-7105 |
DOI: | 10.1038/s41592-022-01688-9 |