Integrating TSPO PET imaging and transcriptomics to unveil the role of neuroinflammation and amyloid-β deposition in Alzheimer’s disease

• Published in: European journal of nuclear medicine and molecular imaging Vol. 51; no. 2; pp. 455 - 467
• Main Authors: Zhang, Miao, Qian, Xiao-hang, Hu, Jialin, Zhang, Yaoyu, Lin, Xiaozhu, Hai, Wangxi, Shi, Kuangyu, Jiang, Xufeng, Li, Yao, Tang, Hui-dong, Li, Biao
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.01.2024; Springer Nature B.V
• Subjects: Alzheimer's disease; Cardiology; CD200 antigen; Cognitive ability; CXCR4 protein; Dementia; Deposition; Fluorine isotopes; Gene expression; Genes; Genetic factors; Genomics; Hospitals; Imaging; Immunization; Immunology; Inflammation; Magnetic resonance imaging; Medical imaging; Medicine; Medicine & Public Health; Microbiota; Microglia; Neurodegenerative diseases; Neuropsychology; Nuclear Medicine; Oncology; Original; Original Article; Orthopedics; Pathogenesis; Positron emission; Positron emission tomography; Radiology; Transcriptomics; β-Amyloid; TSPO; Neuroinflammation; [; Amyloid-β; F]DPA-714 PET/MR; Alzheimer’s disease; CD200; [18F]DPA-714 PET/MR
• ISSN: 1619-7070; 1619-7089
• DOI: 10.1007/s00259-023-06446-3

Purpose Despite the revealed role of immunological dysfunctions in the development and progression of Alzheimer’s disease (AD) through animal and postmortem investigations, direct evidence regarding the impact of genetic factors on microglia response and amyloid-β (Aβ) deposition in AD individuals is lacking. This study aims to elucidate this mechanism by integrating transcriptomics and TSPO, Aβ PET imaging in clinical AD cohort. Methods We analyzed 85 patients with PET/MR imaging for microglial activation (TSPO, [ 18 F]DPA-714) and Aβ ([ 18 F]AV-45) within the prospective Alzheimer’s Disease Immunization and Microbiota Initiative Study Cohort (ADIMIC). Immune-related differentially expressed genes (IREDGs), identified based on AlzData, were screened and verified using blood samples from ADIMIC. Correlation and mediation analyses were applied to investigate the relationships between immune-related genes expression, TSPO and Aβ PET imaging. Results TSPO uptake increased significantly both in aMCI ( P  < 0.05) and AD participants ( P  < 0.01) and showed a positive correlation with Aβ deposition (r = 0.42, P  < 0.001). Decreased expression of TGFBR3, FABP3, CXCR4 and CD200 was observed in AD group. CD200 expression was significantly negatively associated with TSPO PET uptake (r =—0.33, P  = 0.013). Mediation analysis indicated that CD200 acted as a significant mediator between TSPO uptake and Aβ deposition (total effect B = 1.92, P  = 0.004) and MMSE score (total effect B =—54.01, P  = 0.003). Conclusion By integrating transcriptomics and TSPO PET imaging in the same clinical AD cohort, this study revealed CD200 played an important role in regulating neuroinflammation, Aβ deposition and cognitive dysfunction.