Development and characterization of microsatellite primers for Triops granarius (Branchiopoda: Notostraca) using MiSeq technology
Background Next-generation sequencing technology has allowed for the rapid development of microsatellites, neutral polymorphic markers that can be used for the analysis of population structure. Methods and Results In this study, we performed whole-genome sequencing using the Illumina MiSeq system an...
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Published in | Molecular biology reports Vol. 49; no. 10; pp. 10121 - 10125 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English Norwegian |
Published |
Dordrecht
Springer Netherlands
01.10.2022
Springer Nature B.V Kluwer Academic/Plenum Publishers |
Subjects | |
Online Access | Get full text |
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Summary: | Background
Next-generation sequencing technology has allowed for the rapid development of microsatellites, neutral polymorphic markers that can be used for the analysis of population structure.
Methods and Results
In this study, we performed whole-genome sequencing using the Illumina MiSeq system and
de novo
assembly to design microsatellite primers for
Triops granarius
populations in Qatar. The developed microsatellites are suitable for future studies of genetic structuring among geographically isolated freshwater pools. A total of 23 different primer pairs produced typical microsatellite results, with each pair successfully amplified in up to 40 individuals. Only five of the loci produced a significant departure from Hardy-Weinberg equilibrium.
Conclusions
Some of the underlying mechanisms regarding the few loci that deviated from HWE may be further investigated to determine the source of deviation. As
T. granarius
is the most widely distributed species of the family, the development of these molecular markers would be useful for conducting population genetics and biogeographical studies broadly. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0301-4851 1573-4978 |
DOI: | 10.1007/s11033-022-07804-4 |