High expression of Dicer reveals a negative prognostic influence in certain subtypes of primary cutaneous T cell lymphomas

• Published in: Journal of dermatological science Vol. 64; no. 3; pp. 185 - 190
• Main Authors: Valencak, Julia, Schmid, Katharina, Trautinger, Franz, Wallnöfer, Werner, Muellauer, Leonhard, Soleiman, Afschin, Knobler, Robert, Haitel, Andrea, Pehamberger, Hubert, Raderer, Markus
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.12.2011
• Subjects: Adult; Aged; Aged, 80 and over; Austria; Biomarkers, Tumor - analysis; DEAD-box RNA Helicases - analysis; Dermatology; Dicer, microRNA; Disease Progression; Disease-Free Survival; Female; Humans; Immunohistochemistry; Kaplan-Meier Estimate; Lymphoma, Primary Cutaneous Anaplastic Large Cell - enzymology; Lymphoma, T-Cell - enzymology; Lymphoma, T-Cell, Cutaneous - enzymology; Lymphoma, T-Cell, Cutaneous - genetics; Lymphoma, T-Cell, Cutaneous - mortality; Lymphoma, T-Cell, Cutaneous - pathology; Lymphoma, T-Cell, Cutaneous - therapy; Lymphomatoid Papulosis - enzymology; Male; Middle Aged; Mycosis Fungoides - enzymology; Neoplasm Staging; Panniculitis - enzymology; Primary cutaneous T cell lymphomas; Prognostic factor; Proportional Hazards Models; Retrospective Studies; Ribonuclease III - analysis; Risk Assessment; Risk Factors; Sezary Syndrome - enzymology; Skin Neoplasms - enzymology; Skin Neoplasms - genetics; Skin Neoplasms - mortality; Skin Neoplasms - pathology; Skin Neoplasms - therapy; Survival; Survival Rate; Time Factors; Treatment Outcome; Up-Regulation; Austria; Prognostic factor; Primary cutaneous T cell lymphomas; Survival; Dicer, microRNA
• ISSN: 0923-1811; 1873-569X
• DOI: 10.1016/j.jdermsci.2011.08.011

Abstract Background Aberrant expression of microRNAs (miRNAs) has been implicated in oncogenesis of various tumors and primary cutaneous T cell lymphomas. Dicer, a ribonuclease III-like enzyme is essential for miRNA processing. Objective We initiated a retrospective study to characterize the alterations in the expression profile of Dicer in patients with primary cutaneous T cell lymphomas (CTCL). Methods A total of 50 consecutive patients with primary CTCL were studied, with the majority having mycosis fungoides ( n = 34). Five patients had primary cutaneous CD 30+ anaplastic large cell lymphoma, four patients each had lymphomatoid papulosis and primary cutaneous CD4-positive small/medium T-cell lymphoma, one primary cutaneous γδ T cell lymphoma, one Sézary syndrome and another subcutaneous panniculitis-like T cell lymphoma of αβ-phenotype. Immunohistochemistry was performed on paraffin sections using a commercially available antibody against Dicer. Intensity of expression was correlated with clinical parameters including disease specific survival (DSS) and time to progression (TTP). Results After a median follow-up of 74 months (range: 1–271), 12/50 patients (24%) have died. Univariate and multivariate analysis for disease-specific survival showed Dicer expression and stage as a negative predictive factor in the sole group of MF patients ( n = 34) as well as in the heterogeneous group of patients ( n = 50), but not gender, histological subtype, primary localization of disease, age and recurrence of lymphoma ( p > 0.05). Conclusion Our data suggest Dicer expression as a possible molecular marker in patients with MF and apparently indicate that miRNA(s) might be of clinical relevance in CTCL.