Characterization of Local and Systemic Impact of Whitefly ( Bemisia tabaci ) Feeding and Whitefly-Transmitted Tomato Mottle Virus Infection on Tomato Leaves by Comprehensive Proteomics

• Published in: International journal of molecular sciences Vol. 21; no. 19; p. 7241
• Main Authors: Ogden, Aaron J, Boukari, Wardatou, Nava, Alba, Lucinda, Natalia, Sunter, Garry, Curtis, Wayne R, Adkins, Joshua N, Polston, Jane E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.10.2020; MDPI
• Subjects: Aleyrodidae; Animals; Begomovirus; Begomovirus - genetics; Begomovirus - pathogenicity; Bemisia tabaci; Biological activity; Cell cycle; Deoxyribonucleic acid; Disease transmission; Diseases and pests; DNA; DNA methylation; Elongation; Experiments; Feeding; Food and nutrition; Geminivirus; Gene expression; Genomes; Hemiptera - genetics; Hemiptera - virology; Host-virus relationships; Infections; Insect-plant relationships; Leaves; Lycopersicon esculentum - genetics; Lycopersicon esculentum - growth & development; Lycopersicon esculentum - virology; Maturation; mRNA; Observations; Pathogenesis; Physiological aspects; Plant Diseases - genetics; Plant Diseases - virology; Plant Leaves - genetics; Plant Leaves - growth & development; Plant Leaves - virology; Plant Proteins - classification; Plant Proteins - genetics; Plant virus diseases; Plant viruses; Plasmodesmata; Principal components analysis; Proteins; Proteomics; RNA directed DNA methylation; RNA transport; RNA viruses; Single-stranded DNA; Solanum lycopersicum; tomato; tomato mottle virus; Tomatoes; transcriptional gene silencing; Translation elongation; Translation initiation; Variance analysis; Viruses; United States; Florida; United States > US; pathogenesis; proteomics; tomato mottle virus; transcriptional gene silencing; Bemisia tabaci; Geminivirus; whitefly; tomato; Begomovirus; RNA directed DNA methylation
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms21197241

Tomato mottle virus (ToMoV) is a single-stranded DNA (ssDNA) begomovirus transmitted to solanaceous crops by the whitefly species complex ( ), causing stunted growth, leaf mottling, and reduced yield. Using a genetic repertoire of seven genes, ToMoV pathogenesis includes the manipulation of multiple plant biological processes to circumvent antiviral defenses. To further understand the effects of whitefly feeding and whitefly-transmitted ToMoV infection on tomato plants ( 'Florida Lanai'), we generated comprehensive protein profiles of leaves subjected to feeding by either viruliferous whiteflies harboring ToMoV, or non-viruliferous whiteflies, or a no-feeding control. The effects of whitefly feeding and ToMoV infection were measured both locally and systemically by sampling either a mature leaf directly from the site of clip-cage confined whitefly feeding, or from a newly formed leaf 10 days post feeding (dpf). At 3 dpf, tomato's response to ToMoV included proteins associated with translation initiation and elongation as well as plasmodesmata dynamics. In contrast, systemic impacts of ToMoV on younger leaves 10 dpf were more pronounced and included a virus-specific change in plant proteins associated with mRNA maturation and export, RNA-dependent DNA methylation, and other antiviral plant processes. Our analysis supports previous findings and provides novel insight into tomato's local and systemic response to whitefly feeding and ToMoV infection.