Metabolic engineering of Klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production
Objectives To improve the production of 2,3-butanediol (2,3-BD) in Klebsiella pneumoniae , the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated. Results Although the cell growth and 2,3-BD production rates of the K. pneumoniae ΔldhA ΔadhE Δpta - ackA strain wer...
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Published in | Biotechnology letters Vol. 38; no. 6; pp. 975 - 982 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.06.2016
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Objectives
To improve the production of 2,3-butanediol (2,3-BD) in
Klebsiella pneumoniae
, the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated.
Results
Although the cell growth and 2,3-BD production rates of the
K. pneumoniae
ΔldhA
ΔadhE
Δpta
-
ackA
strain were lower than those of the wild-type strain, the mutant produced a higher titer of 2,3-BD and a higher yield in batch fermentation: 91 g 2,3-BD/l with a yield of 0.45 g per g glucose and a productivity of 1.62 g/l.h in fed-batch fermentation. The metabolic characteristics of the mutants were consistent with the results of in silico simulation.
Conclusions
K. pneumoniae
knockout mutants developed with an aid of in silico investigation could produce higher amounts of 2,3-BD with increased titer, yield, and productivity. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0141-5492 1573-6776 |
DOI: | 10.1007/s10529-016-2062-y |