Metabolic engineering of Klebsiella pneumoniae and in silico investigation for enhanced 2,3-butanediol production

• Published in: Biotechnology letters Vol. 38; no. 6; pp. 975 - 982
• Main Authors: Rathnasingh, Chelladurai, Park, Jong Myoung, Kim, Duk-ki, Song, Hyohak, Chang, Yong Keun
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2016; Springer Nature B.V
• Subjects: Acetic acid; Applied Microbiology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Batch Cell Culture Techniques; Biochemistry; Biomedical and Life Sciences; Biotechnology; Butylene Glycols - metabolism; Cell growth; Computer Simulation; Ethanol; Ethyl alcohol; Fermentation; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genes; Glucose; Klebsiella; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - metabolism; Life Sciences; Metabolic Engineering - methods; Microbiology; Mutation; Original Research Paper; Pneumonia; Productivity; Strain; Lactate dehydrogenase; Phosphotransacetylase; Acetate kinase; Alcohol dehydrogenase; In silico investigation; 2,3-Butanediol; Klebsiella pneumoniae
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-016-2062-y

Objectives To improve the production of 2,3-butanediol (2,3-BD) in Klebsiella pneumoniae , the genes related to the formation of lactic acid, ethanol, and acetic acid were eliminated. Results Although the cell growth and 2,3-BD production rates of the K. pneumoniae ΔldhA ΔadhE Δpta - ackA strain were lower than those of the wild-type strain, the mutant produced a higher titer of 2,3-BD and a higher yield in batch fermentation: 91 g 2,3-BD/l with a yield of 0.45 g per g glucose and a productivity of 1.62 g/l.h in fed-batch fermentation. The metabolic characteristics of the mutants were consistent with the results of in silico simulation. Conclusions K. pneumoniae knockout mutants developed with an aid of in silico investigation could produce higher amounts of 2,3-BD with increased titer, yield, and productivity.