Trends in SPR Cytometry: Advances in Label-Free Detection of Cell Parameters

SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers h...

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Published inBiosensors (Basel) Vol. 8; no. 4; p. 102
Main Authors Schasfoort, Richard B M, Abali, Fikri, Stojanovic, Ivan, Vidarsson, Gestur, Terstappen, Leon W M M
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 30.10.2018
MDPI
Subjects
Antibodies
Antigens
Apoptosis
Biosensors
Blood
Cell surface
Cytometry
Ligands
Mammalian cells
Morphology
Parameters
Review
Sensors
single-cell secretion
SPR imaging
Surface plasmon resonance
cytometry
single-cell secretion
surface plasmon resonance
SPR imaging
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Summary:SPR cytometry entails the measurement of parameters from intact cells using the surface plasmon resonance (SPR) phenomenon. Specific real-time and label-free binding of living cells to sensor surfaces has been made possible through the availability of SPR imaging (SPRi) instruments and researchers have started to explore its potential in the last decade. Here we will discuss the mechanisms of detection and additionally describe the problems and issues of mammalian cells in SPR biosensing, both from our own experience and with information from the literature. Finally, we build on the knowledge and applications that has already materialized in this field to give a forecast of some exciting applications for SPRi cytometry.
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ISSN:2079-6374
2079-6374
DOI:10.3390/bios8040102