Complete mitochondrial genome sequence and comparative analysis of the cultivated yellow nutsedge

• Published in: The plant genome Vol. 15; no. 3; pp. e20239 - n/a
• Main Authors: Niu, Lu, Zhang, Yuanyu, Yang, Chunming, Yang, Jing, Ren, Wei, Zhong, Xiaofang, Zhao, Qianqian, Xing, Guojie, Zhao, Yongguo, Yang, Xiangdong
• Format: Journal Article
• Language: English
• Published: Madison John Wiley & Sons, Inc 01.09.2022; Wiley
• Subjects: Angiosperms; Annotations; Comparative analysis; Cultivation; Cyperaceae; Cyperus esculentus; Evolution; Gene clusters; Gene order; Genes; Genomes; Maximum likelihood method; Metabolism; Mitochondria; Mitochondrial DNA; Nucleotide sequence; Phylogenetics; Phylogeny; Plant mitochondria; Plastids; Proteins; rRNA; Software; Sorghum; Species; Synteny; Transfer RNA; tRNA; Tubers
• ISSN: 1940-3372; 1940-3372
• DOI: 10.1002/tpg2.20239

As a monocotyledonous plant in family Cyperaceae, yellow nutsedge (Cyperus esculentus L.) is unique in accumulating a substantial amount of oil in underground tubers and provides a model system for studying oil accumulation in nonseed tissues. However, no data on the mitochondrial and nuclear genome sequences of this species are available, which greatly limits our understanding of its evolutionary characteristics and some essential biological mechanisms. In the present study, we report the first complete mitochondrial genome sequence of the cultivated yellow nutsedge. The analysis of the genome showed that the yellow nutsedge mitochondrial genome is 1,002,696 bp in size and encodes 62 genes consisting of 36 protein‐coding genes (PCGs), 20 transfer RNA (tRNA) genes, and six ribosomal RNA (rRNA) genes. Compared with other angiosperms, yellow nutsedge mitochondrial genome contains much higher percentage of noncoding sequences (95.36%). Sixteen plastid‐derived fragments were identified to be strongly associated with mitochondrial genes including one intact plastid‐related gene (ndhH). Comparative analysis with seven other sequenced plant mitochondrial genomes revealed that two syntenic gene clusters, rps3‐rpl16 and rps12‐nad3, are highly conserved in all plant mitochondrial genomes, and the mitochondrial genome of yellow nutsedge is more similar to those of monocotyledons in the gene order. Phylogenetic analysis based on 13 shared protein‐encoding genes in eight plant species showed that yellow nutsedge is evolutionarily more closely related to monocotyledonary species. Overall, the species‐specific features of the cultivated yellow nutsedge mitochondrial genome provide additional information for the evolutionary and comparative genomic studies in the yellow nutsedge and other Cyperus species of the Cyperaceae family. Core Ideas We present the first complete mitochondrial genome of the cultivated yellow nutsedge. The mitochondrial genome of yellow nutsedge contains much higher percentage of noncoding sequences and exhibits some species‐specific features in the genome size and structure. Phylogenetic analysis shows that yellow nutsedge is evolutionarily more closely related to monocotyledonary species.