Involvement of Nitric Oxide in UVB-Induced Pigmentation in Guinea Pig Skin

• Published in: Pigment cell research Vol. 13; no. 5; pp. 358 - 363
• Main Authors: HORIKOSHI, TOSHIO, NAKAHARA, MICHIO, KAMINAGA, HIROKO, SASAKI, MINORU, UCHIWA, HIDEYO, MIYACHI, YOSHIKI
• Format: Journal Article
• Language: English
• Published: Copenhagen Munksgaard 01.10.2000
• Subjects: Animals; Dihydroxyphenylalanine - metabolism; Enzyme Inhibitors - pharmacology; Erythema; Erythema - diagnostic imaging; Guinea Pigs; Inhibitor; NG-Nitroarginine Methyl Ester - pharmacology; Nitric oxide; Nitric Oxide - metabolism; Nitric Oxide Synthase - antagonists & inhibitors; Pigmentation; Radionuclide Imaging; Skin - metabolism; Skin - pathology; Skin Pigmentation - radiation effects; Ultraviolet B; Ultraviolet Rays
• ISSN: 0893-5785; 1600-0749
• DOI: 10.1034/j.1600-0749.2000.130509.x

Ultraviolet (UV) B irradiation evokes erythema and delayed pigmentation in skin, where a variety of toxic and modulating events are known to be involved. Nitric oxide (NO) is generated from l ‐arginine by NO synthases (NOS). Production of NO is enhanced in response to UVB‐stimulation and has an important role in the development of erythema. NO has recently been demonstrated as a melanogen which stimulates melanocytes in vitro, however, no known in vivo data has been reported to support this finding. In this study, we investigated the contribution of NO with UV‐induced pigmentation in an animal model using an NOS inhibitor. UVB‐induced erythema in guinea pig skin was reduced when an NOS inhibitor, l‐NAME (N‐nitro‐ l‐arginine methylester hydrochloride), was topically applied to the skin daily, beginning 3 days before UVB‐irradiation. Delayed pigmentation and an increased number of DOPA‐positive melanocytes in the skin were markedly suppressed by sequential daily treatment with l‐NAME. Furthermore, melanin content 13 days after UVB‐irradiation was significantly lower in skin treated with l‐NAME than in the controls. In contrast, d‐NAME (N‐nitro‐ d‐arginine methylester hydrochloride), an ineffective isomer of l‐NAME, demonstrated no effect on these UV‐induced skin responses. These results suggest that NO production may contribute to the regulation of UVB‐induced pigmentation.