Identification of the cytochrome P450 enzymes responsible for the ω-hydroxylation of phytanic acid
Patients suffering from Refsum disease have a defect in the α-oxidation pathway which results in the accumulation of phytanic acid in plasma and tissues. Our previous studies have shown that phytanic acid is also a substrate for the ω-oxidation pathway. With the use of specific inhibitors we now sho...
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Published in | FEBS letters Vol. 580; no. 16; pp. 3794 - 3798 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier B.V
10.07.2006
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Subjects | |
Online Access | Get full text |
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Summary: | Patients suffering from Refsum disease have a defect in the α-oxidation pathway which results in the accumulation of phytanic acid in plasma and tissues. Our previous studies have shown that phytanic acid is also a substrate for the ω-oxidation pathway. With the use of specific inhibitors we now show that members of the cytochrome P450 (CYP450) family 4 class are responsible for phytanic acid ω-hydroxylation. Incubations with microsomes containing human recombinant CYP450s (Supersomes™) revealed that multiple CYP450 enzymes of the family 4 class are able to ω-hydroxylate phytanic acid with the following order of efficiency: CYP4F3A
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CYP4F3B
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CYP4F2
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CYP4A11. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1016/j.febslet.2006.05.069 |