Identification and characterization of RED120: A conserved PWI domain protein with links to splicing and 3′-end formation

• Published in: FEBS letters Vol. 581; no. 16; pp. 3087 - 3097
• Main Authors: Fortes, Puri, Longman, Dasa, McCracken, Susan, Ip, Joanna Y., Poot, Raymond, Mattaj, Iain W., Cáceres, Javier F., Blencowe, Benjamin J.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 26.06.2007
• Subjects: 3′-End processing; Amino Acid Sequence; Animals; Antigens, Nuclear - metabolism; Caenorhabditis elegans - genetics; Cell Nucleus - metabolism; Cloning, Molecular; Conserved Sequence; HeLa Cells; Humans; Molecular Sequence Data; Nuclear Matrix-Associated Proteins - metabolism; Nuclear Proteins; Phylogeny; Protein Binding; Protein Structure, Tertiary - genetics; RNA 3' End Processing - genetics; RNA Precursors - metabolism; RNA Splicing - genetics; RNA, Messenger - metabolism; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; RNAi; Sequence Homology, Amino Acid; snRNPs; Splicing; SRm160; RNAi; snRNPs; Splicing; SRm160; 3′-End processing
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2007.05.066

Precursor (pre)-mRNA splicing can impact the efficiency of coupled steps in gene expression. SRm160 (SR-related nuclear matrix protein of 160 kDa), is a splicing coactivator that also functions as a 3′-end cleavage-stimulatory factor. Here, we have identified an evolutionary-conserved SRm160-interacting protein, referred to as hRED120 (for human Arg/Glu/Asp-rich protein of 120 kDa). hRED120 contains a conventional RNA recognition motif and, like SRm160, a PWI nucleic acid binding domain, suggesting that it has the potential to bridge different RNP complexes. Also, similar to SRm160, hRED120 associates with snRNP components, and remains associated with mRNA after splicing. Simultaneous suppression in Caenorhabditis elegans of the ortholog of hRED120 with the orthologs of splicing and 3′-end processing factors results in aberrant growth or developmental defects. These results suggest that RED120 may function to couple splicing with mRNA 3′-end formation.