Inactivation of Human Immunodeficiency Virus Type 1 by Ozone In Vitro

• Published in: Blood Vol. 78; no. 7; pp. 1882 - 1890
• Main Authors: Wells, Keith H., Latino, Joseph, Gavalchin, Jerrie, Poiesz, Bernard J.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.10.1991; The Americain Society of Hematology
• Subjects: AIDS VIRUS; ANIMALS; Antiviral Agents - administration & dosage; Antiviral Agents - pharmacology; BIOCHEMICAL REACTION KINETICS; Biological and medical sciences; BIOLOGICAL MATERIALS; BODY FLUIDS; Body Fluids - microbiology; Cell Line; Dose-Response Relationship, Drug; DOSE-RESPONSE RELATIONSHIPS; Factor VIII - metabolism; HIV Core Protein p24 - analysis; HIV Core Protein p24 - metabolism; HIV Reverse Transcriptase; HIV-1 - drug effects; HIV-1 - physiology; human immunodeficiency virus; Humans; Immunopathology; KINETICS; MAMMALS; MAN; MATERIALS; Medical sciences; MEMBRANE PROTEINS; MICROORGANISMS; ORGANIC COMPOUNDS; OZONE; Ozone - administration & dosage; Ozone - pharmacology; PARASITES; PRIMATES; PROTEINS; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; REACTION KINETICS; RECEPTORS; Reverse Transcriptase Inhibitors; SENSITIVITY; TOXICITY; VERTEBRATES; VIRUSES 560300 > Chemicals Metabolism & Toxicology; Infection; Virus; Human; Ozonization; Transfusion; Viral disease; Retroviridae; Lentivirinae; AIDS; Human immunodeficiency virus; Inactivation; Blood
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V78.7.1882.1882

A device was designed to deliver a constant source of given concentrations of ozone to fluids containing human immunodeficiency virus type 1 (HIV-1). Ozone was found to inactivate HIV-1 virions in a dose-dependent manner. Greater than 11 log inactivation was achieved within 2 hours at a concentration of 1, 200 ppm ozone. Similar concentrations of ozone had minimal effect on factor VIII activity in both plasma and immunoaffinity-purified preparations of factor VIII treated for the same time period. The data indicate that the antiviral effects of ozone include viral particle disruption, reverse transcriptase inactivation, and/or a perturbation of the ability of the virus to bind to its receptor on target cells. Ozone treatment offers promise as a means to inactivate human retroviruses in human body fluids and blood product preparations.