The Association of Vesicular Contents and Its Effects on Release

: The oxidation of catecholamines with a carbon‐fiber electrode can be used to monitor exocytosis at the single cell level at a variety of different types of cells. These measurements allow release to be followed from individual vesicles and have revealed several unique aspects concerning the coupli...

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Bibliographic Details
Published inAnnals of the New York Academy of Sciences Vol. 971; no. 1; pp. 620 - 626
Main Authors WIGHTMAN, R. MARK, TROYER, KEVIN P., MUNDORF, MICHELLE L., CATAHAN, REBECCA
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.10.2002
Subjects
Animals
Calcium - metabolism
carbon-fiber electrode
chromaffin cell
Chromaffin Cells - metabolism
Chromaffin Cells - physiology
chromogranin
cyclic voltammetry
Electrodes
Electrophysiology - methods
Exocytosis
intravesicular Ca21
mast cell
Mast Cells - physiology
Neurons - metabolism
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Summary:: The oxidation of catecholamines with a carbon‐fiber electrode can be used to monitor exocytosis at the single cell level at a variety of different types of cells. These measurements allow release to be followed from individual vesicles and have revealed several unique aspects concerning the coupling between release and storage. The strong association of the vesicular components in chromaffin cells dictates the time course of extrusion of the vesicle contents. Furthermore, liberation of the Ca2+ normally stored within the vesicles can promote exocytosis without an external Ca2+ source.
Bibliography:ark:/67375/WNG-61FGGWQJ-7
ArticleID:NYAS620
istex:15B12981584F94BD918579B72589A423FE5ECA2A
ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-3
content type line 23
ObjectType-Review-1
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.2002.tb04540.x