Nano-Selenium inhibited antibiotic resistance genes and virulence factors by suppressing bacterial selenocompound metabolism and chemotaxis pathways in animal manure

• Published in: Ecotoxicology and environmental safety Vol. 263; p. 115277
• Main Authors: Zhang, Haibo, Zhao, Zhigang, Guan, Weikun, Zhong, Yuhong, Wang, Yang, Zhou, Qilong, Liu, Fuyu, Luo, Qi, Liu, Junyi, Ni, Jian, He, Ning, Guo, Dongsheng, Li, Lizhi, Xing, Qingfeng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.09.2023; Elsevier
• Subjects: Antibiotic resistance genes; Bacterial chemotaxis; Biological nano-Selenium; Broiler manure production; Graphene sodium selenite; Virulence factors; Graphene sodium selenite; Bacterial chemotaxis; Virulence factors; Antibiotic resistance genes; Broiler manure production; Biological nano-Selenium
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2023.115277

Numerous antibiotic resistance genes (ARGs) and virulence factors (VFs) found in animal manure pose significant risks to human health. However, the effects of graphene sodium selenite (GSSe), a novel chemical nano-Selenium, and biological nano-Selenium (BNSSe), a new bioaugmentation nano-Se, on bacterial Se metabolism, chemotaxis, ARGs, and VFs in animal manure remain unknown. In this study, we investigated the effects of GSSe and BNSSe on ARGs and VFs expression in broiler manure using high-throughput sequencing. Results showed that BNSSe reduced Se pressure during anaerobic fermentation by inhibiting bacterial selenocompound metabolism pathways, thereby lowering manure Selenium pollution. Additionally, the expression levels of ARGs and VFs were lower in the BNSSe group compared to the Sodium Selenite and GSSe groups, as BNSSe inhibited bacterial chemotaxis pathways. Co-occurrence network analysis identified ARGs and VFs within the following phyla Bacteroidetes (genera Butyricimonas, Odoribacter, Paraprevotella, and Rikenella), Firmicutes (genera Lactobacillus, Candidatus_Borkfalkia, Merdimonas, Oscillibacter, Intestinimonas, and Megamonas), and Proteobacteria (genera Desulfovibrio). The expression and abundance of ARGs and VFs genes were found to be associated with ARGs-VFs coexistence. Moreover, BNSSe disruption of bacterial selenocompound metabolism and chemotaxis pathways resulted in less frequent transfer of ARGs and VFs. These findings indicate that BNSSe can reduce ARGs and VFs expression in animal manure by suppressing bacterial selenocompound metabolism and chemotaxis pathways. [Display omitted] •Nano-Selenium can affect the expression of ARGs and VFs in vivo using metagenomics.•BNSSe reduced manure Se content and reduces harmful contaminants in the environment.•BNSSe disrupted chemotaxis pathways, minimizing ARG and VFs transfer.•Selenocompound metabolism and chemotaxis pathways determined ARGs-VFs coexistence.