Effect of Sodium Selenite on the Metabolite Profile of Epichloë sp. Mycelia from Festuca sinensis in Solid Culture

• Published in: Biological trace element research Vol. 200; no. 11; pp. 4865 - 4879
• Main Authors: Zhou, Lianyu, Jiao, Lu, Ju, Jiasheng, Ma, Xuelan
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.11.2022; Springer Nature B.V
• Subjects: Agar; Amino acids; Antioxidant properties; Antioxidants - pharmacology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Carbohydrates; Cell culture; Cellobiose; Chromatography; Colonies; Cultivation; Dextrose; Diameters; Epichloe - chemistry; Epichloe - metabolism; Exposure; Fatty Acids; Festuca; Festuca - metabolism; Gas chromatography; Glucose; Glutathione; Grasses; Humans; Isomaltose; Life Sciences; Lysine; Maltose; Metabolites; Micronutrients; Microorganisms; Mycelia; Nutrition; Oncology; Palmitic acid; Palmitic Acids; Palmitoleic acid; Selenious Acid; Selenites; Selenium; Selenium - metabolism; Selenium - pharmacology; Sodium; Sodium selenite; Sodium Selenite - metabolism; Sodium Selenite - pharmacology; Stearic acid; Stearic Acids; Sucrose; Tyrosine; Uridine; sp. from; Selenium; Culture time; Metabolite analysis; Epichloë sp. from Festuca sinensis
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-021-03054-w

Selenium (Se) is an essential micronutrient with many beneficial effects for humans and other living organisms. Numerous microorganisms in culture systems enrich and convert inorganic selenium to organic selenium. In this study, Epichloë sp. from Festuca sinensis was exposed to increasing Na 2 SeO 3 concentrations (0, 0.1, 0.2, 0.3, and 0.4 mmol/L) in Petri dishes with potato dextrose agar (PDA) for 8 weeks. Epichloë sp. mycelia were immediately collected after mycelial diameters were measured at 4, 5, 6, 7, and 8 weeks of cultivation, respectively. Gas chromatography-mass spectrometer (GC-MS) analysis was performed on different groups of Epichloë sp. mycelia. Different changes were observed as Epichloë sp. was exposed to different selenite conditions and cultivation time. The colony diameter of Epichloë sp. decreased in response to increased selenite concentrations, whereas the inhibitory effects diminished over time. Seventy-two of the 203 identified metabolites did not differ significantly across selenite treatments within the same time point, while 82 compounds did not differ significantly between multiple time points of the same Se concentration. However, the relative levels of 122 metabolites increased the most under selenite conditions. Specifically, between the 4th and 8th weeks, there were increases in 2-keto-isovaleric acid, uridine, and maltose in selenite treatments compared to controls. Selenium increased glutathione levels and exhibited antioxidant properties in weeks 4, 5, and 7. Additionally, we observed that different doses of selenite could promote the production of carbohydrates such as isomaltose, cellobiose, and sucrose; fatty acids such as palmitoleic acid, palmitic acid, and stearic acid; and amino acids such as lysine and tyrosine in Epichloë sp. mycelia. Therefore, Epichloë sp. exposed to selenite stress may benefit from increased levels of some metabolite compounds.