Identification of casein peptides in plasma of subjects after a cheese-enriched diet

• Published in: Food research international Vol. 84; pp. 108 - 112
• Main Authors: Caira, Simonetta, Pinto, Gabriella, Vitaglione, Paola, Dal Piaz, Fabrizio, Ferranti, Pasquale, Addeo, Francesco
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.06.2016
• Subjects: absorption; Bioavailability; Casein; Cheese; Cheese phosphopeptides; chromatography; dairy consumption; Diets; fasting; Human blood; Hydroxyapatite; Hydroxyapatite chromatography; Intakes; oligopeptides; Parmesan cheese; Peptides; Pilots; Tandem mass spectrometry; Hydroxyapatite chromatography; Tandem mass spectrometry; Cheese phosphopeptides; Human blood
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2016.03.023

Despite several studies support the functionality of casein peptides in vitro, a gap of knowledge still exist about their bioavailability. In this pilot study the bioavailability of phosphopeptides (CPPs) was investigated in four healthy subjects who consumed for one week 100g/day of Parmigiano Reggiano cheese after one week of a dairy products-free diet. CPPs were detected in plasma samples from fasting subjects after the cheese-enriched diet and peptides were detected variously in almost all the 4 samples. Some αs1- and αs2-CN-derived CPPs, such as αs1-CN (f43–52 and f43–50) and αs2-CN (f8–12), (f7–12) and (f6–12) as well as four non-phosphorylated peptides belonging to the C-terminal end of β-CN (f193–209, f194–209, f200–209) were detected in plasma samples submitted to extraction and enrichment by hydroxyapatite (HA) chromatography followed by MALDI-TOF and nano LC-ESI/MS/MS analysis. Data indicated that casein oligopeptides are bioavailable after a continued intake of cheese. Future studies are warranted to ascertain this finding on a wider population and to clarify the mechanisms behind CPPs bioaccessibility and absorption. [Display omitted] •Enrichment of phosphopeptide by hydroxyapaptite-based chromatography from human serum;•First detection of specific casein peptides and CPPs in the blood stream persons after Parmigiano Reggiano cheese intake;•Characterization of bovine peptides detected in human blood by MS/MS analysis;•Tracking the origin of bovine peptides of human blood from the cheese source.