Effects of a Combination of Arginine Silicate Inositol Complex and a Novel Form of Biotin on Hair and Nail Growth in a Rodent Model

• Published in: Biological trace element research Vol. 201; no. 2; pp. 751 - 765
• Main Authors: Demir, Betul, Cicek, Demet, Orhan, Cemal, Er, Besir, Erten, Fusun, Tuzcu, Mehmet, Ozercan, Ibrahim Hanifi, Sahin, Nurhan, Komorowski, James, Ojalvo, Sara Perez, Sylla, Sarah, Sahin, Kazim
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2023; Springer Nature B.V
• Subjects: Animal models; Animals; Arginine; Arginine - pharmacology; beta Catenin; Biochemistry; Biomedical and Life Sciences; Biotechnology; Biotin; Blood pressure; Dermatitis; Diet; Female; Fibroblast growth factor 2; Glucose; Hair; Inositol; Inositol - pharmacology; Inositols; Insulin-Like Growth Factor I; Insulin-like growth factors; Laboratory animals; Life Sciences; Magnesium; Magnesium compounds; Metabolism; Nutrition; Oncology; Rats; Rats, Sprague-Dawley; Rodentia; Rodents; Silicates; Silicates - pharmacology; Skin; Tumor necrosis factor-TNF; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A; Vitamin B complex; Wnt protein; β-Catenin; Hair; Nail; Biotin; Magnesium; Arginine silicate
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-022-03176-9

The purpose of this study was to examine the effects of a combination of inositol-stabilized arginine silicate complex (ASI) and magnesium biotinate (MgB) on hair and nail growth in an animal model. Twenty-eight female Sprague–Dawley rats (8 weeks old) were randomized into one of the following groups: (i) group (control), shaved; (ii) group (ASI), shaved + ASI (4.14 mg/rat/day); (iii) group (ASI + MgB I), shaved + ASI (4.14 mg/rat/day) + MgB (48.7 μg/rat/day); and (iv) group (ASI + MgB II), shaved + ASI (4.14 mg/rat/day) + MgB (325 μg/rat/day). On day 42, compared with the control group, while hair density ( p  < 0.05, p  < 0.01, and p  < 0.0001, respectively) and anagen ratio ( p  < 0.01, p  < 0.01, and p  < 0.001) increased in the ASI, ASI + MgB I, and ASI + MgB II groups, telogen ratio decreased ( p  < 0.01, p  < 0.01, and p  < 0.001, respectively). In the molecular analysis, VEGF, HGF, and KGF-2 increased in the ASI ( p  < 0.01, p  < 0.01, and p  < 0.05, respectively), ASI + MgB I ( p  < 0.0001 for all), and ASI + MgB II ( p  < 0.0001 for all) groups when compared to the control group. FGF-2 ( p  < 0.01) and IGF-1 ( p  < 0.001) were found to be increased in the ASI + MgB I and ASI + MgB II groups. SIRT-1 and β-catenin increased in the ASI ( p  < 0.05 and p  < 0.01), ASI + MgB I ( p  < 0.001 for both), and ASI + MgB II ( p  < 0.0001 for both) groups. Wnt-1 increased in the ASI + MgB I ( p  < 0.001) and ASI + MgB II ( p  < 0.0001) groups. In conclusion , the combination of ASI and MgB could promote hair growth by regulating IGF-1, FGF, KGF, HGF, VEGF, SIRT-1, Wnt, and β-catenin signal pathways. It was also established that ASI did not affect nail growth, whereas the MgB combination was effective using a higher dose of biotin.