Transcriptome analysis in the thiamethoxam resistance of seven-spot ladybird beetle, Coccinella septempunctata (Coleoptera: Coccinellidae)

• Published in: Ecotoxicology and environmental safety Vol. 230; p. 113144
• Main Authors: Cheng, Zhi, Wang, Da, Han, Shipeng, Zuo, Cheng, He, Yunzhuan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15.01.2022; Elsevier
• Subjects: Coccinella septempunctata; Cytochrome P450 monooxygenase; Nicotinic acetylcholine receptor; Resistance; Thiamethoxam; Resistance; Thiamethoxam; Coccinella septempunctata; Cytochrome P450 monooxygenase; Nicotinic acetylcholine receptor
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.113144

The seven-spot ladybird beetle, Coccinella septempunctata Linnaeus (Coleoptera: Coccinellidae) has been used as the main biological control agent against all kinds of aphids in farmland and greenhouse. In this study, a thiamethoxam-resistant strain (ThR) and a susceptible strain (SS) of seven-spot ladybird beetle were established, and differentially expressed genes (DEGs) associated with thiamethoxam resistance were recorded through de novo Illumina HiSeq 4000 sequencing. A total of 53.5 Gb of clean data were obtained and finally assembled into 21,217 unigenes from ThR and SS transcriptomes. 1798 DEGs were identified between the ThR libraries and the SS libraries, including 560 up-regulated genes and 1238 down-regulated genes. Some cytochrome p450 monooxygenases (CYP450s), UDP-glycosyltransferases (UGTs), esterases (ESTs) and ATP-binding cassette (ABC) transporters were observed to be up-regulated and the nicotinic acetylcholine receptors (nAChRs) α subunit gene down-regulated in the ThR strain compared to the SS strain. This study provides genetic information for further studies on thiamethoxam resistance mechanisms in the seven-spot ladybird beetle. •Sensitive and resistant populations of non-target insect Coccinella septempunctata were established.•Resistance mechanism of Coccinella septempunctata to thiamethoxam may be related to the up regulation of detoxification gene.•The potential effects of thiamethoxam on non-target insect Coccinella septempunctata were revealed.